Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGA   Type   Machinery gene
Locus tag   MCCS_RS07110 Genome accession   NZ_CP021059
Coordinates   1365630..1366601 (-) Length   323 a.a.
NCBI ID   WP_086042732.1    Uniprot ID   -
Organism   Macrococcoides canis strain KM 45013     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1315419..1365679 1365630..1366601 flank -49


Gene organization within MGE regions


Location: 1315419..1366601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCCS_RS06735 (MCCS_13860) - 1315419..1316246 (-) 828 WP_086042658.1 SH3 domain-containing protein -
  MCCS_RS06740 (MCCS_13870) - 1316248..1316514 (-) 267 WP_086042659.1 phage holin -
  MCCS_RS06745 (MCCS_13880) - 1316560..1316946 (-) 387 WP_086042660.1 hypothetical protein -
  MCCS_RS12610 - 1317177..1317398 (+) 222 WP_157891064.1 hypothetical protein -
  MCCS_RS06755 (MCCS_13910) - 1317587..1318222 (-) 636 WP_086042662.1 hypothetical protein -
  MCCS_RS06760 (MCCS_13920) - 1318377..1320413 (-) 2037 WP_086042663.1 NosD domain-containing protein -
  MCCS_RS06765 (MCCS_13930) - 1320400..1320684 (-) 285 WP_086042664.1 hypothetical protein -
  MCCS_RS06770 (MCCS_13940) - 1320695..1322488 (-) 1794 WP_086042665.1 phage tail spike protein -
  MCCS_RS06775 (MCCS_13950) - 1322488..1322922 (-) 435 WP_086042666.1 hypothetical protein -
  MCCS_RS06780 (MCCS_13960) - 1322915..1328569 (-) 5655 WP_086042667.1 LysM peptidoglycan-binding domain-containing protein -
  MCCS_RS06785 (MCCS_13970) - 1328609..1328992 (-) 384 WP_086042668.1 hypothetical protein -
  MCCS_RS06790 (MCCS_13980) - 1328947..1329468 (-) 522 WP_086042669.1 hypothetical protein -
  MCCS_RS06795 (MCCS_13990) - 1329546..1330136 (-) 591 WP_086042670.1 hypothetical protein -
  MCCS_RS06800 (MCCS_14000) - 1330200..1330580 (-) 381 WP_086042671.1 minor capsid protein -
  MCCS_RS06805 (MCCS_14010) - 1330585..1331067 (-) 483 WP_157891065.1 HK97 gp10 family phage protein -
  MCCS_RS12615 (MCCS_14020) - 1331057..1331389 (-) 333 WP_157891066.1 hypothetical protein -
  MCCS_RS06810 (MCCS_14030) - 1331401..1331784 (-) 384 WP_086042673.1 hypothetical protein -
  MCCS_RS06815 (MCCS_14040) - 1331802..1332077 (-) 276 WP_086042674.1 hypothetical protein -
  MCCS_RS06820 (MCCS_14050) - 1332119..1333123 (-) 1005 WP_086042675.1 major capsid protein -
  MCCS_RS06825 (MCCS_14060) - 1333150..1333524 (-) 375 WP_086042676.1 hypothetical protein -
  MCCS_RS06830 (MCCS_14070) - 1333537..1334142 (-) 606 WP_086042677.1 phage scaffolding protein -
  MCCS_RS06835 (MCCS_14080) - 1334397..1334588 (-) 192 WP_086042678.1 hypothetical protein -
  MCCS_RS06840 (MCCS_14090) - 1334605..1334862 (-) 258 WP_086042679.1 hypothetical protein -
  MCCS_RS06845 (MCCS_14100) - 1334849..1335070 (-) 222 WP_086042680.1 hypothetical protein -
  MCCS_RS06850 (MCCS_14110) - 1335159..1336235 (-) 1077 WP_086042681.1 phage minor capsid protein -
  MCCS_RS06855 (MCCS_14120) - 1336247..1336684 (-) 438 WP_086042682.1 hypothetical protein -
  MCCS_RS06860 (MCCS_14130) - 1336668..1338308 (-) 1641 WP_086042683.1 hypothetical protein -
  MCCS_RS06865 (MCCS_14140) - 1338321..1339613 (-) 1293 WP_086042684.1 PBSX family phage terminase large subunit -
  MCCS_RS06870 (MCCS_14150) - 1339610..1340323 (-) 714 WP_086042685.1 hypothetical protein -
  MCCS_RS06875 (MCCS_14160) - 1340489..1340992 (-) 504 WP_157891067.1 HNH endonuclease -
  MCCS_RS06880 (MCCS_14170) - 1341168..1342040 (-) 873 WP_086042687.1 hypothetical protein -
  MCCS_RS06885 (MCCS_14180) - 1342107..1342511 (-) 405 WP_086042688.1 hypothetical protein -
  MCCS_RS06890 (MCCS_14190) - 1342528..1343013 (-) 486 WP_086042689.1 Holliday junction resolvase RecU -
  MCCS_RS06895 (MCCS_14200) dut 1343056..1343478 (-) 423 WP_086042690.1 dUTP diphosphatase -
  MCCS_RS06900 (MCCS_14210) - 1343459..1343641 (-) 183 WP_086042691.1 hypothetical protein -
  MCCS_RS06905 (MCCS_14220) - 1343631..1344110 (-) 480 WP_086042692.1 hypothetical protein -
  MCCS_RS06910 (MCCS_14230) - 1344111..1344383 (-) 273 WP_157891068.1 hypothetical protein -
  MCCS_RS06915 (MCCS_14240) - 1344376..1344750 (-) 375 WP_086042694.1 hypothetical protein -
  MCCS_RS12620 (MCCS_14250) - 1344747..1344920 (-) 174 WP_157891069.1 hypothetical protein -
  MCCS_RS06920 (MCCS_14270) - 1345005..1345208 (-) 204 WP_086042695.1 hypothetical protein -
  MCCS_RS06930 (MCCS_14290) - 1345545..1345769 (-) 225 WP_086042697.1 hypothetical protein -
  MCCS_RS06935 (MCCS_14300) - 1345853..1346284 (-) 432 WP_086042698.1 hypothetical protein -
  MCCS_RS06940 (MCCS_14310) - 1346384..1346587 (-) 204 WP_086042699.1 hypothetical protein -
  MCCS_RS06945 (MCCS_14320) - 1346578..1346976 (-) 399 WP_086042700.1 YopX family protein -
  MCCS_RS06950 (MCCS_14330) - 1346973..1347245 (-) 273 WP_086042701.1 hypothetical protein -
  MCCS_RS06955 (MCCS_14340) - 1347259..1347462 (-) 204 WP_086042702.1 hypothetical protein -
  MCCS_RS06960 (MCCS_14350) - 1347476..1347745 (-) 270 WP_167625963.1 hypothetical protein -
  MCCS_RS06965 (MCCS_14360) - 1347742..1348965 (-) 1224 WP_086042704.1 DUF3310 domain-containing protein -
  MCCS_RS06970 (MCCS_14370) - 1348968..1349195 (-) 228 WP_086042705.1 helix-turn-helix domain-containing protein -
  MCCS_RS06975 (MCCS_14380) - 1349198..1349590 (-) 393 WP_086042706.1 hypothetical protein -
  MCCS_RS06980 (MCCS_14390) - 1349603..1349782 (-) 180 WP_157891070.1 hypothetical protein -
  MCCS_RS06985 (MCCS_14400) - 1349779..1350000 (-) 222 WP_086042708.1 hypothetical protein -
  MCCS_RS06990 (MCCS_14410) - 1349997..1350275 (-) 279 WP_086042709.1 hypothetical protein -
  MCCS_RS06995 (MCCS_14420) - 1350288..1350650 (-) 363 WP_086042710.1 YkgJ family cysteine cluster protein -
  MCCS_RS07000 (MCCS_14430) ssbA 1350663..1351169 (-) 507 WP_086042711.1 single-stranded DNA-binding protein Machinery gene
  MCCS_RS07005 (MCCS_14440) - 1351162..1351683 (-) 522 WP_086042712.1 HNH endonuclease -
  MCCS_RS07010 (MCCS_14450) - 1351729..1351923 (-) 195 WP_086042713.1 hypothetical protein -
  MCCS_RS12625 (MCCS_14460) - 1351920..1352096 (-) 177 WP_157891071.1 hypothetical protein -
  MCCS_RS12630 (MCCS_14470) - 1352068..1352244 (-) 177 WP_157891072.1 hypothetical protein -
  MCCS_RS12635 (MCCS_14480) - 1352241..1352417 (-) 177 WP_157891073.1 hypothetical protein -
  MCCS_RS07015 (MCCS_14500) - 1352535..1353317 (-) 783 WP_167625964.1 ATP-binding protein -
  MCCS_RS07020 (MCCS_14510) - 1353298..1354176 (-) 879 WP_086042715.1 phage replisome organizer N-terminal domain-containing protein -
  MCCS_RS07025 (MCCS_14520) - 1354190..1354906 (-) 717 WP_086042716.1 MBL fold metallo-hydrolase -
  MCCS_RS07030 (MCCS_14530) bet 1354906..1355658 (-) 753 WP_086042717.1 phage recombination protein Bet -
  MCCS_RS07035 (MCCS_14540) - 1355660..1357636 (-) 1977 WP_086042718.1 AAA family ATPase -
  MCCS_RS07040 (MCCS_14550) - 1357636..1357818 (-) 183 WP_086042719.1 hypothetical protein -
  MCCS_RS07045 (MCCS_14560) - 1357815..1358582 (-) 768 WP_086042720.1 phage regulatory protein/antirepressor Ant -
  MCCS_RS07050 (MCCS_14580) - 1358668..1358907 (-) 240 WP_086042721.1 hypothetical protein -
  MCCS_RS07055 (MCCS_14590) - 1358908..1359099 (-) 192 WP_086042722.1 hypothetical protein -
  MCCS_RS07060 (MCCS_14600) - 1359101..1359346 (-) 246 WP_086042723.1 hypothetical protein -
  MCCS_RS07065 (MCCS_14610) - 1359360..1359863 (-) 504 WP_086042724.1 hypothetical protein -
  MCCS_RS07070 (MCCS_14620) - 1359979..1360230 (-) 252 WP_086042725.1 helix-turn-helix transcriptional regulator -
  MCCS_RS07075 (MCCS_14630) - 1360384..1360725 (+) 342 WP_086042726.1 helix-turn-helix transcriptional regulator -
  MCCS_RS12640 (MCCS_14640) - 1360745..1360912 (+) 168 WP_157891074.1 hypothetical protein -
  MCCS_RS07080 (MCCS_14650) - 1360920..1361819 (+) 900 WP_157891075.1 DUF4352 domain-containing protein -
  MCCS_RS12745 (MCCS_14660) - 1361846..1362628 (+) 783 WP_193432083.1 hypothetical protein -
  MCCS_RS07090 (MCCS_14670) - 1362746..1363207 (+) 462 WP_086042728.1 ImmA/IrrE family metallo-endopeptidase -
  MCCS_RS07095 (MCCS_14680) - 1363211..1364425 (+) 1215 WP_086042729.1 site-specific integrase -
  MCCS_RS07100 - 1364513..1364593 (-) 81 WP_086042730.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MCCS_RS07105 (MCCS_14690) comGB 1364765..1365655 (-) 891 WP_167625965.1 competence type IV pilus assembly protein ComGB -
  MCCS_RS07110 (MCCS_14700) comGA 1365630..1366601 (-) 972 WP_086042732.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence


Protein


Download         Length: 323 a.a.        Molecular weight: 37257.85 Da        Isoelectric Point: 6.7240

>NTDB_id=228024 MCCS_RS07110 WP_086042732.1 1365630..1366601(-) (comGA) [Macrococcoides canis strain KM 45013]
MEKLFNEIIEEAIIQGASDIHFIPNDKSISIKFRIHGDIEDYREIDDMLFRKLLSYIKFTAHLDVSEKNKAQSGIIHFNL
KDVRYNIRASTLPRALGDEACVLRIIRQNFIDEYQTDDKILFNQIKKSSGIIIISGPTGSGKSTLMYQLVHYARNTLKRQ
IISIEDPVEQHIEGIIQVNVNEKAEITYHTAIKAILRCDPDIIMLGEIRDSVVAQQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINTVDLYQSINLIINQRLVRKKDGKGRLLTYEFLTKKDIEKYLQGEHINYRTLTDQFKELYETNQISHYEFEK
FDL

Nucleotide


Download         Length: 972 bp        

>NTDB_id=228024 MCCS_RS07110 WP_086042732.1 1365630..1366601(-) (comGA) [Macrococcoides canis strain KM 45013]
ATGGAAAAATTATTCAATGAAATAATAGAGGAAGCAATTATACAAGGTGCATCAGATATTCACTTCATACCAAACGATAA
AAGCATATCTATCAAATTTAGGATACATGGTGATATTGAAGACTATAGAGAAATCGACGACATGTTATTCAGGAAATTAC
TTTCTTATATTAAATTTACGGCACATCTTGATGTATCAGAAAAGAATAAGGCACAGAGTGGAATCATCCATTTTAATCTG
AAAGACGTAAGATATAATATTCGCGCATCTACTTTACCTCGAGCATTAGGCGATGAAGCATGTGTACTGAGAATCATCAG
ACAAAATTTTATAGATGAATATCAGACGGATGACAAGATATTGTTCAATCAAATAAAAAAATCAAGCGGTATTATCATCA
TAAGTGGACCAACTGGAAGCGGGAAAAGCACATTGATGTATCAGCTTGTACATTATGCGAGGAACACATTGAAGAGACAA
ATAATATCAATAGAAGATCCTGTTGAACAGCATATTGAAGGAATTATACAAGTTAATGTTAACGAAAAAGCAGAGATAAC
ATATCATACCGCGATAAAGGCAATCCTTAGATGTGATCCGGATATCATTATGCTAGGTGAAATCAGAGATTCAGTCGTAG
CACAGCAAGTGATCAATGCAGGACTAAGCGGTCATCTCGTTTTAACGACATTACATGCGAATGATTGCATCGGTGCATTA
TTTCGGTTAAAAGAAATGGGTATTAATACTGTTGATCTATATCAAAGTATCAATCTGATTATTAATCAGAGGCTTGTAAG
AAAGAAAGATGGGAAGGGGCGTCTATTGACCTATGAATTTTTGACAAAAAAGGATATTGAAAAATATTTACAAGGTGAAC
ATATTAACTATAGAACGTTGACCGATCAATTTAAGGAGCTCTATGAAACAAATCAGATTTCACACTATGAATTCGAAAAA
TTTGATCTATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

48.78

100

0.495

  comGA Staphylococcus aureus N315

48.78

100

0.495

  comGA Bacillus subtilis subsp. subtilis str. 168

38.037

100

0.384

  pilB Glaesserella parasuis strain SC1401

34.012

100

0.362

  pilB Vibrio parahaemolyticus RIMD 2210633

34.513

100

0.362


Multiple sequence alignment