Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   B6N60_RS14565 Genome accession   NZ_CP021056
Coordinates   3462469..3464481 (-) Length   670 a.a.
NCBI ID   WP_190601385.1    Uniprot ID   -
Organism   Richelia sinica FACHB-800 strain FACHB 800     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3457469..3469481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6N60_RS14545 (B6N60_03227) bioU 3458218..3459225 (+) 1008 WP_190601381.1 (S)-8-amino-7-oxononanoate synthase BioU -
  B6N60_RS14550 (B6N60_03228) - 3459311..3459871 (-) 561 WP_190601382.1 hypothetical protein -
  B6N60_RS14555 (B6N60_03229) - 3459951..3461174 (-) 1224 WP_190601383.1 type II secretion system F family protein -
  B6N60_RS14560 (B6N60_03230) pilT 3461288..3462409 (-) 1122 WP_190601384.1 type IV pilus twitching motility protein PilT Machinery gene
  B6N60_RS14565 (B6N60_03231) pilB/pilB1 3462469..3464481 (-) 2013 WP_190601385.1 GspE/PulE family protein Machinery gene
  B6N60_RS14570 (B6N60_03232) grpE 3465094..3465840 (+) 747 WP_190601564.1 nucleotide exchange factor GrpE -
  B6N60_RS14575 (B6N60_03233) dnaK 3466155..3468146 (+) 1992 WP_190601386.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 75067.91 Da        Isoelectric Point: 5.1194

>NTDB_id=227967 B6N60_RS14565 WP_190601385.1 3462469..3464481(-) (pilB/pilB1) [Richelia sinica FACHB-800 strain FACHB 800]
MTYSSQPRRSTALTTRTEFSPFGNKLVQSGYVNNEQMRQALIESRKSGRPLTDVLESITGRQLSPEFLREYKKQHLFELK
ILYGVESLDPEVNQVGTAMVMTLIETLIPVDICRRHRLVPLSKHEDQNPPSVLVAMVDPDNLEASDDLNRILRPQGLALQ
RMVITQEDYQKLINQYLDELAVRQKQLEQEKFTDIQQDLENLESLDMDDSGEDMDVDLTAAMKDAEDAPIISLVNRILAK
ALQDKVSDIHIEPQEENLRIRFRKDGVLREAFPPVPKKVIPAVTARFKIISNLDIAERRLPQDGRIRRLYEGRKVDFRVN
TLPSRYGEKVCLRILDNSSTQLGLNKLITDPETLQIVKDMVSKPFGLILVTGPTGSGKTTSLYSALAEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSDCRIPYTPTTEELARYGLTAAQETSVTFYKANVVPPESLAEAKSRNQVCQTCGG
VGYKGRCGVYEVMRITENLQTLINQEAPTERIKEVAVEEGMKTLLAYSLDLVRQGSTTLEEVERVTFTDTGLEAELKARR
KSGLTCRTCSAELKPEWLDCPYCLTPRFTD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=227967 B6N60_RS14565 WP_190601385.1 3462469..3464481(-) (pilB/pilB1) [Richelia sinica FACHB-800 strain FACHB 800]
ATGACTTACTCGTCACAACCAAGGCGCAGCACCGCTCTAACCACACGAACAGAGTTTTCTCCCTTCGGTAATAAGCTAGT
TCAATCTGGCTATGTCAATAACGAACAGATGCGGCAAGCACTGATAGAAAGCCGTAAATCTGGTAGGCCGTTGACAGATG
TATTAGAGTCTATTACTGGCAGACAACTCTCACCAGAGTTTCTGAGGGAATACAAAAAACAGCATTTATTTGAACTCAAA
ATCCTCTACGGGGTTGAGTCCCTTGACCCAGAAGTCAATCAAGTCGGCACGGCAATGGTGATGACTTTGATTGAAACCCT
CATTCCTGTGGATATCTGTCGTCGTCATCGCTTGGTGCCATTATCCAAGCATGAAGACCAAAACCCACCTTCTGTGTTGG
TGGCGATGGTTGATCCAGATAACTTGGAAGCCTCTGATGATTTGAATCGGATTTTGCGTCCTCAAGGCCTAGCTTTGCAA
AGGATGGTCATCACCCAGGAGGATTACCAAAAACTAATTAATCAATATCTAGATGAGTTAGCAGTCCGCCAAAAACAACT
AGAGCAGGAAAAGTTCACAGACATTCAACAAGATTTAGAGAATCTGGAAAGTCTGGACATGGACGATAGTGGGGAGGATA
TGGATGTTGATCTGACCGCAGCGATGAAGGATGCAGAAGATGCACCCATCATCAGTCTGGTCAATCGCATCTTAGCTAAA
GCCCTGCAAGATAAGGTGTCAGATATTCATATCGAACCACAAGAAGAAAACTTACGTATTCGCTTTCGCAAAGATGGGGT
ATTGCGTGAAGCTTTTCCCCCTGTACCCAAAAAGGTTATTCCCGCAGTTACAGCCCGATTTAAAATTATTTCTAACCTAG
ACATTGCAGAACGACGTTTACCTCAGGATGGACGTATTCGCCGTCTGTATGAAGGCAGAAAGGTAGACTTTCGGGTCAAT
ACCTTACCCAGTCGCTACGGGGAAAAGGTATGTTTGCGGATTTTGGATAATTCCTCTACTCAATTAGGGTTGAATAAGTT
AATTACTGATCCAGAAACTTTGCAAATAGTCAAAGATATGGTTAGTAAACCATTCGGGTTGATTCTCGTCACAGGCCCTA
CTGGTTCTGGTAAAACCACTTCTTTGTATTCTGCATTGGCAGAAAAGAACGACCCAGGCATTAACATCAGTACGGTAGAA
GACCCAATTGAGTATAGTTTGCCGGGGATTACTCAAGTACAAGTAATTCGGGAAAAAGGGTTGGATTTTGCAACAGCATT
AAGGGCATTCTTACGACAAGATCCAGATGTGCTACTGGTGGGTGAAACACGGGATAAGGAAACCGCCAAAACTGCGATTG
AAGCTGCGCTTACAGGACACTTAGTATTAACAACTTTACACACTAATGATGCTCCAGGCGCGATCGCTCGCTTGGGAGAA
ATGGGAATTGAGCCTTTCATGGTTTCCAGTTCTCTGATTGGTGTATTAGCACAACGTCTGGTGCGTCGTGTATGTTCTGA
TTGTCGTATCCCCTATACTCCCACTACAGAAGAACTAGCTCGCTACGGTCTCACTGCTGCCCAAGAAACAAGTGTTACTT
TTTACAAAGCTAATGTTGTGCCACCAGAGTCCTTAGCAGAAGCTAAATCCAGAAATCAAGTTTGTCAAACTTGCGGAGGA
GTTGGTTATAAGGGACGTTGCGGTGTGTATGAAGTAATGCGGATCACCGAAAACCTCCAAACCTTGATTAACCAAGAAGC
ACCCACAGAACGCATCAAGGAAGTAGCCGTTGAAGAAGGGATGAAAACCTTGTTGGCTTACAGTCTGGATTTAGTTCGTC
AAGGTTCTACCACCTTAGAAGAAGTAGAACGGGTAACATTCACTGATACCGGTTTGGAAGCTGAATTAAAAGCCAGACGT
AAAAGCGGCTTGACATGTCGGACTTGTAGTGCCGAATTAAAACCAGAGTGGTTAGATTGTCCCTACTGTCTGACACCTCG
ATTTACAGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-179)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.785

100

0.651

Multiple sequence alignment