Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   B6N60_RS14560 Genome accession   NZ_CP021056
Coordinates   3461288..3462409 (-) Length   373 a.a.
NCBI ID   WP_190601384.1    Uniprot ID   A0A975Y5R8
Organism   Richelia sinica FACHB-800 strain FACHB 800     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3456288..3467409
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6N60_RS14530 (B6N60_03224) - 3456438..3456887 (-) 450 WP_190601379.1 HNH endonuclease -
  B6N60_RS14535 (B6N60_03225) - 3456893..3457345 (-) 453 WP_244997441.1 hypothetical protein -
  B6N60_RS14540 (B6N60_03226) - 3457346..3457930 (-) 585 WP_190601380.1 hypothetical protein -
  B6N60_RS14545 (B6N60_03227) bioU 3458218..3459225 (+) 1008 WP_190601381.1 (S)-8-amino-7-oxononanoate synthase BioU -
  B6N60_RS14550 (B6N60_03228) - 3459311..3459871 (-) 561 WP_190601382.1 hypothetical protein -
  B6N60_RS14555 (B6N60_03229) - 3459951..3461174 (-) 1224 WP_190601383.1 type II secretion system F family protein -
  B6N60_RS14560 (B6N60_03230) pilT 3461288..3462409 (-) 1122 WP_190601384.1 type IV pilus twitching motility protein PilT Machinery gene
  B6N60_RS14565 (B6N60_03231) pilB/pilB1 3462469..3464481 (-) 2013 WP_190601385.1 GspE/PulE family protein Machinery gene
  B6N60_RS14570 (B6N60_03232) grpE 3465094..3465840 (+) 747 WP_190601564.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 40819.06 Da        Isoelectric Point: 7.4416

>NTDB_id=227966 B6N60_RS14560 WP_190601384.1 3461288..3462409(-) (pilT) [Richelia sinica FACHB-800 strain FACHB 800]
MELMIEDLMEQLIEMGGSDLHLSAGLPPYFRISGKLTPIGDQPLTADQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGTYAACLRALSSKIPNFEKLGLPDVVREMSEKPRGLILVTGPTGSGKTTTLAAMIDLINRTRAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIILVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHEKQTQVRVQLSNSLVAVFSQTLVPKKNPKPGEYGRVMAQEIMVITPAISNLIREGKTSQIYSAIQTGAKLGM
QTLEKVLADLYKNGSISFEAAMSKTSKPDEVQRLIGSAAPPAAAKSGAASRAH

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=227966 B6N60_RS14560 WP_190601384.1 3461288..3462409(-) (pilT) [Richelia sinica FACHB-800 strain FACHB 800]
ATGGAATTGATGATTGAAGATTTGATGGAGCAGTTGATTGAAATGGGTGGTTCTGACTTACATCTATCCGCAGGATTGCC
CCCCTATTTTCGGATTAGTGGTAAATTAACTCCCATTGGTGATCAACCCCTCACTGCTGATCAATGCCAAAGACTGATTT
TTAGTATGCTCAACAACACCCAGCGTAAAACCTTAGAACAAACCTGGGAATTAGATTGTTCCTATGGTGTGAAAGGATTA
GCTCGCTTTCGGGTGAACGTCTATAAAGAACGTGGCACATATGCAGCTTGTTTACGAGCATTAAGTTCTAAAATTCCTAA
CTTTGAAAAACTAGGTCTACCGGATGTAGTACGGGAAATGTCGGAAAAGCCTAGAGGCTTAATTCTCGTCACTGGCCCCA
CAGGCTCAGGAAAAACCACTACCTTGGCGGCCATGATTGATTTAATTAACCGCACAAGAGCCGAACATATTCTCACGGTA
GAAGACCCAATTGAATTTGTTTACGAACCCATTAAAAGCCTAGTTCACCAACGTCAACTGGGTGAAGATACTAAGAGTTT
TGCTAATGCTTTAAAAGCGGCTTTACGGGAAGACCCAGATATTATTCTGGTAGGGGAAATGCGGGATTTAGAAACAATTT
CCTTGGCTATTTCTGCGGCAGAAACAGGTCACTTAGTATTTGGTACATTACACACTAGTTCGGCAGCCCAAACTGTTGAC
CGGATTATTGACGTTTTCCCCCATGAAAAACAAACCCAAGTTAGGGTGCAGTTGTCTAACTCCCTTGTGGCAGTATTCAG
CCAAACCCTAGTACCGAAGAAAAACCCCAAACCAGGGGAATATGGTCGAGTCATGGCTCAAGAAATCATGGTAATCACTC
CTGCTATTTCTAACCTGATTCGTGAAGGTAAAACATCACAAATTTACTCAGCTATCCAAACTGGAGCTAAATTAGGGATG
CAAACTTTGGAGAAAGTGTTAGCAGATTTGTATAAAAATGGCTCTATATCTTTTGAAGCAGCAATGTCTAAGACTTCTAA
ACCTGATGAAGTCCAACGCTTAATTGGTTCTGCGGCTCCTCCGGCAGCAGCAAAATCTGGTGCAGCATCCAGAGCACATT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.362

92.493

0.512
  pilT Pseudomonas stutzeri DSM 10701

54.203

92.493

0.501
  pilT Legionella pneumophila strain ERS1305867

54.203

92.493

0.501
  pilT Legionella pneumophila strain Lp02

54.203

92.493

0.501
  pilT Acinetobacter baylyi ADP1

53.623

92.493

0.496
  pilT Acinetobacter baumannii D1279779

53.333

92.493

0.493
  pilT Acinetobacter baumannii strain A118

53.333

92.493

0.493
  pilT Acinetobacter nosocomialis M2

53.333

92.493

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.619

100

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.588

91.153

0.461
  pilT Vibrio cholerae strain A1552

50.588

91.153

0.461
  pilT Neisseria gonorrhoeae MS11

50.442

90.885

0.458
  pilT Neisseria meningitidis 8013

50.147

90.885

0.456

Multiple sequence alignment