Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   B6257_RS07220 Genome accession   NZ_CP021011
Coordinates   1378675..1380060 (-) Length   461 a.a.
NCBI ID   WP_101562487.1    Uniprot ID   -
Organism   Bacillus velezensis strain GFP-2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1373675..1385060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6257_RS07185 (B6257_07150) flgL 1373850..1374767 (-) 918 WP_003151396.1 flagellar hook-associated protein FlgL -
  B6257_RS07190 (B6257_07155) flgK 1374779..1376296 (-) 1518 WP_015387629.1 flagellar hook-associated protein FlgK -
  B6257_RS07195 (B6257_07160) - 1376312..1376794 (-) 483 WP_003151394.1 flagellar protein FlgN -
  B6257_RS07200 (B6257_07165) flgM 1376809..1377075 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  B6257_RS07205 (B6257_07170) - 1377145..1377564 (-) 420 WP_003151392.1 TIGR03826 family flagellar region protein -
  B6257_RS07210 (B6257_07175) comFC 1377638..1378327 (-) 690 WP_199445943.1 phosphoribosyltransferase family protein Machinery gene
  B6257_RS07215 (B6257_07180) - 1378333..1378617 (-) 285 WP_017418816.1 late competence development ComFB family protein -
  B6257_RS07220 (B6257_07185) comFA 1378675..1380060 (-) 1386 WP_101562487.1 DEAD/DEAH box helicase Machinery gene
  B6257_RS07225 (B6257_07190) - 1380168..1381016 (-) 849 WP_101562488.1 DegV family protein -
  B6257_RS07230 (B6257_07195) degU 1381114..1381803 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  B6257_RS07235 (B6257_07200) degS 1381880..1383043 (-) 1164 WP_003151385.1 two-component sensor histidine kinase DegS Regulator
  B6257_RS07240 (B6257_07205) - 1383266..1383913 (+) 648 WP_060657503.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52569.94 Da        Isoelectric Point: 10.0531

>NTDB_id=227803 B6257_RS07220 WP_101562487.1 1378675..1380060(-) (comFA) [Bacillus velezensis strain GFP-2]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRINRFIEKEYITAEPSIIRRKNRYVCQRCGQSDQACFAAFWAPSAKRQTT
YCRACVMMGRADELTSLYSWNQAPENSWEPVKLSWEGTLTDGQKQAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVDVSALYGSSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DEILRFAVDKARKKNSALVYVTATPSDTLKKNAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQSQLPVFLFVPSLHVLKKTTDYFQKLNIRAEGVHAEDTFRKDKVKRFRDGLLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGMTNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=227803 B6257_RS07220 WP_101562487.1 1378675..1380060(-) (comFA) [Bacillus velezensis strain GFP-2]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
TTCAGATCAGCGCATCAACCGTTTTATAGAAAAAGAGTATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCTGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAGAGGCAGACTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAGCCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCAGGCCGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCTGGTATCGAGTTT
GCCCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGACGTTTCTGCTTTGTACGGCAGCAGCGAGGACAAAGGCCGCTTGACTCCGCTGATGATTTCAA
CCGCTCATCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGATATTAAGGTTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAATGCTGAAGCCGGTTTGCTGAAAAGCGTTCGCATACCGGCCAGATATCATCGGAAACCTCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATCACAGCTGCCGGTTTTCTTATTCGTTCCGTCTTTACACGTATTGAAAAAGACAACGGATTATTT
TCAGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGGTTCAGAGACG
GCCTGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATGACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(118-266)

Helicase conserved C-terminal domain (Helicase_C)

(329-421)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.174

99.783

0.62

Multiple sequence alignment