Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   B9L64_RS05095 Genome accession   NZ_CP020915
Coordinates   1025423..1026040 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain 50-1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1020423..1031040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9L64_RS05070 rpsT 1020724..1020990 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  B9L64_RS05075 holA 1021005..1022048 (-) 1044 WP_015714286.1 DNA polymerase III subunit delta -
  B9L64_RS20960 - 1022088..1022237 (-) 150 WP_003229985.1 hypothetical protein -
  B9L64_RS05080 yqzM 1022278..1022412 (+) 135 WP_003229983.1 YqzM family protein -
  B9L64_RS05085 comEC 1022453..1024783 (-) 2331 WP_069704087.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  B9L64_RS05090 comEB 1024787..1025356 (-) 570 WP_003229978.1 ComE operon protein 2 -
  B9L64_RS05095 comEA 1025423..1026040 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  B9L64_RS05100 comER 1026124..1026945 (+) 822 WP_014480313.1 late competence protein ComER -
  B9L64_RS05105 yqeM 1027011..1027754 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  B9L64_RS05110 rsfS 1027751..1028107 (-) 357 WP_014480315.1 ribosome silencing factor -
  B9L64_RS05115 yqeK 1028125..1028685 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  B9L64_RS05120 nadD 1028675..1029244 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  B9L64_RS05125 yhbY 1029256..1029546 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  B9L64_RS05130 aroE 1029540..1030382 (-) 843 WP_041351695.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=227069 B9L64_RS05095 WP_004398514.1 1025423..1026040(-) (comEA) [Bacillus subtilis strain 50-1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=227069 B9L64_RS05095 WP_004398514.1 1025423..1026040(-) (comEA) [Bacillus subtilis strain 50-1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment