Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   B9C53_RS17370 Genome accession   NZ_CP020874
Coordinates   3548737..3549351 (+) Length   204 a.a.
NCBI ID   WP_038459268.1    Uniprot ID   -
Organism   Bacillus velezensis strain GYL4     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3543737..3554351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B9C53_RS17335 (B9C53_17280) aroE 3544423..3545265 (+) 843 WP_003152856.1 shikimate dehydrogenase -
  B9C53_RS17340 (B9C53_17285) yhbY 3545259..3545549 (+) 291 WP_007408257.1 ribosome assembly RNA-binding protein YhbY -
  B9C53_RS17345 (B9C53_17290) - 3545560..3546129 (+) 570 WP_007408258.1 nicotinate-nucleotide adenylyltransferase -
  B9C53_RS17350 (B9C53_17295) yqeK 3546119..3546694 (+) 576 WP_032873949.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  B9C53_RS17355 (B9C53_17300) rsfS 3546698..3547054 (+) 357 WP_007408260.1 ribosome silencing factor -
  B9C53_RS17360 (B9C53_17305) - 3547051..3547788 (+) 738 WP_038459270.1 class I SAM-dependent DNA methyltransferase -
  B9C53_RS17365 (B9C53_17310) comER 3547857..3548678 (-) 822 WP_012118027.1 late competence protein ComER -
  B9C53_RS17370 (B9C53_17315) comEA 3548737..3549351 (+) 615 WP_038459268.1 helix-hairpin-helix domain-containing protein Machinery gene
  B9C53_RS17375 (B9C53_17320) - 3549418..3549987 (+) 570 WP_038459266.1 ComE operon protein 2 -
  B9C53_RS17380 (B9C53_17325) comEC 3549988..3552339 (+) 2352 WP_038459264.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  B9C53_RS17385 (B9C53_17330) - 3552358..3552492 (-) 135 WP_003152870.1 YqzM family protein -
  B9C53_RS19565 - 3552533..3552684 (+) 152 Protein_3340 hypothetical protein -
  B9C53_RS17390 (B9C53_17335) holA 3552724..3553765 (+) 1042 Protein_3341 DNA polymerase III subunit delta -
  B9C53_RS17395 (B9C53_17340) rpsT 3553782..3554048 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22206.44 Da        Isoelectric Point: 6.4939

>NTDB_id=226798 B9C53_RS17370 WP_038459268.1 3548737..3549351(+) (comEA) [Bacillus velezensis strain GYL4]
MTKAMMTEPLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELKVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREEKGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=226798 B9C53_RS17370 WP_038459268.1 3548737..3549351(+) (comEA) [Bacillus velezensis strain GYL4]
ATGACGAAAGCGATGATGACGGAGCCGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAAAGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAAAGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(68-122)

Helix-hairpin-helix motif (HHH_3)

(141-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.115

93.627

0.544
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.071

100

0.363

Multiple sequence alignment