Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C2R14_RS16440 Genome accession   NZ_CP026349
Coordinates   3169827..3170486 (+) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain 6A     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3164827..3175486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2R14_RS16410 ygiS 3166110..3167717 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  C2R14_RS16420 mqsA 3167850..3168245 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  C2R14_RS16425 mqsR 3168247..3168543 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  C2R14_RS16430 ygiV 3168748..3169230 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  C2R14_RS16435 ygiW 3169283..3169675 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C2R14_RS16440 ciaR 3169827..3170486 (+) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  C2R14_RS16445 qseC 3170483..3171832 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  C2R14_RS16450 ygiZ 3171878..3172210 (-) 333 WP_000917684.1 DUF2645 family protein -
  C2R14_RS16460 mdaB 3172529..3173110 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C2R14_RS16465 ygiN 3173141..3173455 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  C2R14_RS16470 parE 3173503..3175395 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=226049 C2R14_RS16440 WP_001221495.1 3169827..3170486(+) (ciaR) [Escherichia coli strain 6A]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=226049 C2R14_RS16440 WP_001221495.1 3169827..3170486(+) (ciaR) [Escherichia coli strain 6A]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361