Detailed information NTG0225967

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: B7941_RS14985
• Genome accession: NZ_CP020805
• Coordinates: 3049650..3050264 (-)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 9D-6
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3044650..3055264

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7941_RS14960 (B7941_14945)	rpsT	3044953..3045219 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
B7941_RS14965 (B7941_14950)	holA	3045236..3046277 (-)	1042	Protein_2931	DNA polymerase III subunit delta	-
B7941_RS19885	-	3046317..3046468 (-)	152	Protein_2932	hypothetical protein	-
B7941_RS14970 (B7941_14955)	-	3046509..3046643 (+)	135	WP_003152870.1	YqzM family protein	-
B7941_RS14975 (B7941_14960)	comEC	3046662..3049013 (-)	2352	WP_085342206.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
B7941_RS14980 (B7941_14965)	-	3049014..3049583 (-)	570	WP_003152868.1	ComE operon protein 2	-
B7941_RS14985 (B7941_14970)	comEA	3049650..3050264 (-)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
B7941_RS14990 (B7941_14975)	comER	3050323..3051144 (+)	822	WP_085342207.1	late competence protein ComER	-
B7941_RS14995 (B7941_14980)	-	3051213..3051950 (-)	738	WP_015240247.1	class I SAM-dependent DNA methyltransferase	-
B7941_RS15000 (B7941_14985)	rsfS	3051947..3052303 (-)	357	WP_007408260.1	ribosome silencing factor	-
B7941_RS15005 (B7941_14990)	yqeK	3052321..3052881 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
B7941_RS15010 (B7941_14995)	-	3052871..3053440 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
B7941_RS15015 (B7941_15000)	yhbY	3053451..3053741 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
B7941_RS15020 (B7941_15005)	aroE	3053735..3054577 (-)	843	WP_025285019.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=225967 B7941_RS14985 WP_015240245.1 3049650..3050264(-) (comEA) [Bacillus velezensis strain 9D-6]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=225967 B7941_RS14985 WP_015240245.1 3049650..3050264(-) (comEA) [Bacillus velezensis strain 9D-6]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGGAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363