Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   B7473_RS03335 Genome accession   NZ_CP020713
Coordinates   644568..645065 (-) Length   165 a.a.
NCBI ID   WP_029694050.1    Uniprot ID   -
Organism   Staphylococcus aureus strain K17     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 639568..650065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7473_RS03310 (B7473_03315) gcvPB 639730..641202 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  B7473_RS03315 (B7473_03320) gcvPA 641195..642541 (-) 1347 WP_000019690.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  B7473_RS03320 (B7473_03325) gcvT 642561..643652 (-) 1092 WP_000093348.1 glycine cleavage system aminomethyltransferase GcvT -
  B7473_RS03325 (B7473_03330) - 643811..644335 (-) 525 WP_001015120.1 shikimate kinase -
  B7473_RS03330 (B7473_03335) - 644325..644471 (-) 147 WP_001792109.1 hypothetical protein -
  B7473_RS03335 (B7473_03340) comGF 644568..645065 (-) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  B7473_RS03340 (B7473_03345) comGE 644983..645282 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  B7473_RS03345 (B7473_03350) comGD 645269..645715 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  B7473_RS03350 (B7473_03355) comGC 645693..646004 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  B7473_RS03355 (B7473_03360) comGB 646018..647088 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  B7473_RS03360 (B7473_03365) comGA 647060..648034 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  B7473_RS03365 (B7473_03370) - 648086..648709 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  B7473_RS03370 (B7473_03375) - 648706..649035 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  B7473_RS03375 (B7473_03380) - 649035..650021 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19199.92 Da        Isoelectric Point: 10.2192

>NTDB_id=225443 B7473_RS03335 WP_029694050.1 644568..645065(-) (comGF) [Staphylococcus aureus strain K17]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIELRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=225443 B7473_RS03335 WP_029694050.1 644568..645065(-) (comGF) [Staphylococcus aureus strain K17]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAACTTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CTTCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.97

100

0.97
  comGF Staphylococcus aureus N315

96.97

100

0.97

Multiple sequence alignment