Detailed information NTG0225338

Bioinformatically predicted

Overview

• Name: pilJ
• Type: Machinery gene
• Locus tag: B5D77_RS04230
• Genome accession: NZ_CP020664
• Coordinates: 821289..823952 (+)
• Length: 887 a.a.
• NCBI ID: WP_106908340.1
• Uniprot ID: A0A2P1UCI3
• Organism: Microcystis sp. MC19
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 816289..828952

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B5D77_RS04205 (B5D77_04160)	hmpF	816408..818048 (-)	1641	WP_106908339.1	pilus motility taxis protein HmpF	-
B5D77_RS26150	-	818972..820011 (+)	1040	Protein_793	RNA-guided endonuclease InsQ/TnpB family protein	-
B5D77_RS04220 (B5D77_04175)	pilH	820208..820573 (+)	366	WP_008202346.1	response regulator transcription factor	Machinery gene
B5D77_RS04225 (B5D77_04180)	-	820665..821195 (+)	531	WP_008202348.1	chemotaxis protein CheW	-
B5D77_RS04230 (B5D77_04185)	pilJ	821289..823952 (+)	2664	WP_106908340.1	methyl-accepting chemotaxis protein	Machinery gene
B5D77_RS04235 (B5D77_04190)	-	824026..825237 (+)	1212	WP_008202352.1	glycosyltransferase	-
B5D77_RS04240 (B5D77_04195)	-	825473..825820 (+)	348	WP_002757647.1	rubredoxin	-
B5D77_RS04245 (B5D77_04200)	-	825927..826919 (+)	993	WP_008202356.1	photosynthesis system II assembly factor Ycf48	-
B5D77_RS04250 (B5D77_04205)	psbE	826998..827243 (+)	246	WP_008202359.1	cytochrome b559 subunit alpha	-
B5D77_RS04255 (B5D77_04210)	psbF	827269..827403 (+)	135	WP_002736175.1	cytochrome b559 subunit beta	-
B5D77_RS04260 (B5D77_04215)	-	827414..827533 (+)	120	WP_002735805.1	photosystem II reaction center protein L	-
B5D77_RS04265 (B5D77_04220)	-	827576..827695 (+)	120	WP_002736108.1	photosystem II reaction center protein J	-

Sequence

Protein

• Length: 887 a.a.
• Molecular weight: 96419.19 Da
• Isoelectric Point: 4.3794

>NTDB_id=225338 B5D77_RS04230 WP_106908340.1 821289..823952(+) (pilJ) [Microcystis sp. MC19]
MPSGTEYAKLYGKANTAYCQGNLEDAALIVKEMSSKYPEDANVILLQGHIHLGLQQYSLAEERYETVLRLAKNSTSRSDL
VDYARRGLDQIRQLGFEVEGGDFTIAATESIAYSEAAFDPGQGFKHSRSGKSQGADPDWQNKGMDWNSSLFNEEDPGEPT
VSKVHSYDDDFEESEETGFSSLTLTSLGSSDSQRSDDSVGGEPPFSFLEAAEIDSLPEEWDGELGSTGEATFMVSSDLGE
NPLTGRDQGLSYSDTPEESAFAIDRQSTQGQGAKTGLNQGRVTQNRYQEEDTGDFRGPELNDEDLDSFSPLGLTDRMQGL
GESELFTRLGEEALSVGFVPEPSGLLNASSGVGEPSFNPSRLKIPNDKRDSHLAHGSDRLLKPVVEVNPGKLAFFVNASL
GKKQLILAALAGITPVILIFAVSTTSWLSTLLAAKPAPNSWAKTEKVAPKPAPASLSPFSQPVLTMMLLTGLGTLAVTWL
GLQLLISQIKRSLNDLQSQFDYLSEGNVNTQATIYSEDEFGQLANRFNQMARVVVTTTTEAQRRAAETEREREDLQRQVI
RLLDDVEGAARGDLTVEAEVSADVLGAVADAFNLTIQNLREIVRQVKKAAKQVNQGSTNSESFARNQSSDALRMAEELAV
TLNSVQMMTDSIQRVAENAREAEEVARTSSITALKGGDSVERTVAGILQIRDTVSETARKVKRLAEASQEISKIVAVVSQ
IASRTNLLALNASIQAARAGDAGRGFAVVADEVRQLADRAAKSLKEIEQIVLQIQSETGSVMMAMEEGIQQVIDVTERSE
QAKKSLEDIIQVSNRIDTLVRSITADTVKQRENSLNVAQVMQSVELTAQETSQESQRVAGSLQKLVSISRELLSSVERFK
VDSEDDR

Nucleotide

• Length: 2664 bp

>NTDB_id=225338 B5D77_RS04230 WP_106908340.1 821289..823952(+) (pilJ) [Microcystis sp. MC19]
ATGCCGTCAGGGACAGAATACGCGAAACTCTATGGAAAGGCTAACACCGCCTATTGCCAGGGCAACCTTGAGGATGCCGC
CCTCATCGTCAAAGAGATGAGCAGCAAGTATCCCGAGGATGCCAATGTCATCCTTTTACAGGGCCACATTCATCTAGGAC
TACAACAATACAGCTTGGCAGAGGAACGTTACGAAACGGTGCTGCGATTGGCTAAAAATTCAACCAGTCGCTCCGATCTG
GTGGATTATGCCAGACGAGGTCTCGATCAGATTCGGCAATTGGGTTTTGAAGTCGAGGGGGGAGATTTTACGATCGCTGC
TACGGAAAGCATTGCCTACAGCGAGGCAGCTTTCGATCCGGGTCAAGGTTTTAAGCATTCTCGGTCTGGAAAGTCGCAAG
GGGCTGACCCCGATTGGCAAAATAAAGGGATGGATTGGAATAGTAGTCTTTTCAATGAGGAGGATCCCGGCGAGCCTACG
GTCAGTAAAGTCCATTCCTATGACGATGATTTTGAAGAATCGGAGGAAACCGGCTTTAGTTCCCTTACCCTAACCAGTTT
AGGTTCCTCCGATTCCCAAAGGTCTGACGATTCTGTGGGGGGGGAACCCCCTTTCTCCTTTTTAGAAGCGGCGGAGATTG
ACTCGCTGCCGGAAGAGTGGGACGGTGAGTTAGGGTCAACGGGAGAGGCCACTTTTATGGTTTCCTCTGACCTGGGGGAA
AATCCCCTCACCGGTCGAGATCAGGGGTTATCCTATAGCGATACCCCAGAAGAAAGCGCTTTTGCCATCGACCGACAATC
GACCCAGGGACAAGGGGCAAAAACTGGCCTTAATCAGGGCAGAGTCACTCAAAACAGATACCAAGAGGAAGACACTGGAG
ATTTTAGGGGACCTGAACTCAATGATGAGGATTTAGACAGTTTTTCCCCACTGGGTCTGACGGATAGGATGCAAGGACTG
GGGGAATCGGAGCTATTTACCCGTTTGGGAGAGGAAGCTTTAAGTGTTGGTTTTGTCCCCGAACCCAGTGGGCTCCTTAA
TGCCAGTTCTGGTGTAGGAGAGCCTAGTTTTAATCCTTCTCGTCTAAAAATCCCCAATGACAAAAGAGATAGTCATCTTG
CCCATGGTTCCGATCGCTTGCTCAAGCCAGTGGTGGAAGTCAATCCGGGCAAATTAGCCTTTTTTGTCAATGCTTCTTTG
GGCAAAAAACAACTAATCCTAGCGGCTTTAGCGGGCATTACCCCTGTAATCTTAATTTTTGCCGTTAGTACCACCTCTTG
GCTTTCTACGCTCTTGGCTGCCAAACCAGCCCCGAATAGCTGGGCAAAAACCGAAAAAGTTGCCCCCAAACCAGCCCCCG
CCAGCCTTTCTCCTTTTAGTCAACCGGTACTAACCATGATGTTACTAACCGGACTGGGAACCCTGGCCGTGACTTGGCTA
GGTTTACAGTTGCTCATTAGTCAGATTAAGCGCAGTCTCAACGATTTGCAAAGCCAATTCGATTATCTCTCGGAAGGTAA
TGTTAACACTCAAGCGACGATCTACTCGGAAGATGAATTCGGTCAACTGGCCAATCGTTTCAATCAGATGGCCCGGGTGG
TGGTAACTACCACCACCGAGGCCCAACGTCGCGCTGCCGAAACGGAACGGGAACGGGAAGACCTACAACGGCAGGTGATT
CGACTGCTCGATGATGTGGAAGGAGCGGCTCGGGGGGATCTAACCGTGGAGGCGGAGGTAAGCGCCGATGTTTTAGGGGC
GGTGGCCGATGCTTTTAATTTGACTATCCAAAACCTGCGGGAAATTGTCCGACAGGTAAAAAAAGCGGCCAAACAGGTGA
ATCAAGGCAGTACCAATAGTGAATCTTTCGCCCGCAATCAGTCTAGCGATGCCCTGCGGATGGCGGAGGAATTGGCCGTC
ACCCTTAATTCGGTACAGATGATGACCGACTCAATTCAACGGGTGGCAGAAAACGCCAGAGAAGCCGAAGAAGTCGCCCG
CACTTCTTCGATTACCGCCCTGAAGGGGGGCGATTCCGTGGAAAGAACCGTGGCGGGAATTCTGCAAATCCGCGACACCG
TATCAGAAACGGCCCGAAAAGTCAAGCGTTTGGCGGAAGCTTCCCAGGAAATCTCGAAAATTGTCGCGGTAGTCTCTCAG
ATTGCCTCGCGCACTAATTTATTGGCTCTTAATGCCTCGATTCAAGCGGCGCGAGCAGGAGATGCGGGCCGCGGTTTTGC
GGTGGTGGCCGATGAGGTGCGTCAGTTGGCCGATAGAGCCGCTAAATCTCTTAAGGAAATTGAACAGATCGTTTTACAGA
TTCAATCGGAAACGGGTTCGGTAATGATGGCAATGGAGGAGGGGATTCAACAGGTTATCGATGTCACCGAGCGCTCGGAA
CAGGCCAAGAAGTCCCTAGAAGATATTATCCAAGTTTCTAACCGCATCGATACGCTGGTGCGCTCGATTACCGCGGATAC
GGTCAAACAACGGGAAAATTCCCTCAATGTCGCCCAAGTTATGCAGTCGGTGGAGTTAACCGCTCAGGAAACTTCCCAAG
AATCCCAACGGGTGGCCGGTTCTCTGCAAAAATTGGTTAGTATCTCCCGGGAGCTGCTTTCTTCAGTAGAACGGTTTAAG
GTAGACTCGGAAGATGATCGATAA

Domains

Predicted by InterproScan.

• Methyl-accepting chemotaxis protein (MCP) signalling domain (MCPsignal) (664-844)

• HAMP domain (HAMP) (484-532)

3D structure

• AlphaFold DB: A0A2P1UCI3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilJ	Synechocystis sp. PCC 6803	49.84	100	0.526