Detailed information NTG0022532

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: SPYM18_RS00330
• Genome accession: NC_003485
• Coordinates: 54497..55495 (+)
• Length: 332 a.a.
• NCBI ID: WP_023079556.1
• Uniprot ID: Q8P302
• Organism: Streptococcus pyogenes MGAS8232
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 49497..60495

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPYM18_RS00315 (spyM18_0035)	-	50224..51564 (+)	1341	WP_011017237.1	hypothetical protein	-
SPYM18_RS00320 (spyM18_0037)	purB	51933..53225 (+)	1293	WP_011017238.1	adenylosuccinate lyase	-
SPYM18_RS00325 (spyM18_0038)	comR	53359..54270 (+)	912	WP_002986681.1	transcriptional regulator Rgg4/ComR	Regulator
SPYM18_RS10730	comS	54355..54450 (+)	96	WP_198462994.1	quorum-sensing system DWW-type pheromone	Regulator
SPYM18_RS00330 (spyM18_0039)	ruvB	54497..55495 (+)	999	WP_023079556.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
SPYM18_RS00335 (spyM18_0040)	-	55633..56070 (+)	438	WP_002994668.1	low molecular weight protein-tyrosine-phosphatase	-
SPYM18_RS00340 (spyM18_0041)	-	56093..56494 (+)	402	WP_011017240.1	MORN repeat-containing protein	-
SPYM18_RS00345 (spyM18_0042)	-	56491..58266 (+)	1776	WP_011017241.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37564.13 Da
• Isoelectric Point: 5.4769

>NTDB_id=22532 SPYM18_RS00330 WP_023079556.1 54497..55495(+) (ruvB) [Streptococcus pyogenes MGAS8232]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLNYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=22532 SPYM18_RS00330 WP_023079556.1 54497..55495(+) (ruvB) [Streptococcus pyogenes MGAS8232]
ATGGCTAGAATTTTAGACAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCTATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTACTATATAGTGCTATGGAAGACTTTTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGATTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCAATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGGCCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACTCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: Q8P302

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.545	99.398	0.84
ruvB	Streptococcus pneumoniae D39	84.545	99.398	0.84
ruvB	Streptococcus pneumoniae TIGR4	84.242	99.398	0.837
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.514	97.289	0.569
ruvB	Helicobacter pylori 26695	48.632	99.096	0.482
ruvB	Synechocystis sp. PCC 6803	49.84	94.277	0.47