Detailed information NTG0225039

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: C2U48_RS18735
• Genome accession: NZ_CP026202
• Coordinates: 3487632..3488291 (-)
• Length: 219 a.a.
• NCBI ID: WP_001221493.1
• Uniprot ID: Q3YXL4
• Organism: Escherichia coli strain ECONIH5
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3482632..3493291

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C2U48_RS18710 (C2U48_18695)	ygiN	3483533..3483847 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
C2U48_RS18715 (C2U48_18700)	mdaB	3483878..3484459 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
C2U48_RS33660	-	3484709..3485188 (+)	480	WP_000065333.1	Hcp family type VI secretion system effector	-
C2U48_RS33665	-	3485191..3485901 (+)	711	WP_000834029.1	hypothetical protein	-
C2U48_RS18725 (C2U48_18710)	ygiZ	3485908..3486240 (+)	333	WP_000914691.1	DUF2645 family protein	-
C2U48_RS18730 (C2U48_18715)	qseC	3486286..3487635 (-)	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
C2U48_RS18735 (C2U48_18720)	ciaR	3487632..3488291 (-)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
C2U48_RS18740 (C2U48_18725)	ygiW	3488443..3488835 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
C2U48_RS18745 (C2U48_18730)	ygiV	3488888..3489370 (+)	483	WP_000609258.1	AraC family transcriptional regulator	-
C2U48_RS18750 (C2U48_18735)	mqsR	3489575..3489871 (+)	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	-
C2U48_RS18755 (C2U48_18740)	mqsA	3489873..3490268 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
C2U48_RS18765 (C2U48_18750)	ygiS	3490401..3492008 (+)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24687.63 Da
• Isoelectric Point: 6.9850

>NTDB_id=225039 C2U48_RS18735 WP_001221493.1 3487632..3488291(-) (ciaR) [Escherichia coli strain ECONIH5]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=225039 C2U48_RS18735 WP_001221493.1 3487632..3488291(-) (ciaR) [Escherichia coli strain ECONIH5]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: Q3YXL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365