Detailed information NTG0224581

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: B7L44_RS20585
• Genome accession: NZ_CP020588
• Coordinates: 3902052..3902912 (+)
• Length: 286 a.a.
• NCBI ID: WP_083009329.1
• Uniprot ID: A0A2L1VKF8
• Organism: Acinetobacter nosocomialis strain SSA3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3897052..3907912

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L44_RS19000 (B7L44_19000)	secG	3897659..3897988 (-)	330	WP_002156913.1	preprotein translocase subunit SecG	-
B7L44_RS19005 (B7L44_19005)	tpiA	3898001..3898795 (-)	795	WP_083009326.1	triose-phosphate isomerase	-
B7L44_RS19010 (B7L44_19010)	pilB	3899086..3900798 (+)	1713	WP_004710829.1	type IV-A pilus assembly ATPase PilB	Machinery gene
B7L44_RS19015 (B7L44_19015)	pilC	3900826..3902052 (+)	1227	WP_083009328.1	type II secretion system F family protein	Machinery gene
B7L44_RS20585 (B7L44_19020)	pilD	3902052..3902912 (+)	861	WP_083009329.1	prepilin peptidase	Machinery gene
B7L44_RS19025 (B7L44_19025)	coaE	3902914..3903510 (+)	597	WP_000874125.1	dephospho-CoA kinase	-
B7L44_RS19030 (B7L44_19030)	-	3903507..3904421 (-)	915	WP_006580951.1	DMT family transporter	-
B7L44_RS19035 (B7L44_19035)	rlmB	3904457..3905206 (-)	750	WP_083009331.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-
B7L44_RS19040 (B7L44_19040)	-	3905308..3905634 (-)	327	WP_000099685.1	pyrimidine/purine nucleoside phosphorylase	-
B7L44_RS19045 (B7L44_19045)	-	3905711..3907021 (-)	1311	WP_083009332.1	tetratricopeptide repeat protein	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 32121.54 Da
• Isoelectric Point: 7.1785

>NTDB_id=224581 B7L44_RS20585 WP_083009329.1 3902052..3902912(+) (pilD) [Acinetobacter nosocomialis strain SSA3]
MQEIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPRMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPACHQP
IRWYQNIPLISWLVLRGKCGHCQHPISIRYPAIELLTMLCSLVVVMVFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDR
FTLPLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSL
LGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG

Nucleotide

• Length: 861 bp

>NTDB_id=224581 B7L44_RS20585 WP_083009329.1 3902052..3902912(+) (pilD) [Acinetobacter nosocomialis strain SSA3]
ATGCAAGAAATCATTGCGTATTTTATTCAAAACTTAACTGCACTTTATATTGCTGTTGCACTCGTGAGCCTATGTATCGG
TAGCTTTCTGAATGTAGTGATTTACCGCACGCCAAGAATGATGGAGCAAGATTGGCAGCAAGAATGCCAAATGTTACTCA
ATCCTGAGCAACCCATTATTGATCATGAGAAGCTAACTTTAAGTAAGCCTGCTTCATCATGCCCTGCATGCCATCAACCC
ATCCGTTGGTATCAAAATATCCCACTCATAAGCTGGCTAGTGTTAAGAGGAAAATGTGGTCATTGCCAGCACCCGATTAG
CATTCGCTATCCAGCCATCGAACTACTTACCATGCTATGTTCATTGGTGGTGGTTATGGTGTTTGGTCCAACCATACAAA
TGCTTTTTGGACTCGTCCTCACATGGGTATTGATAGCACTTACCTTTATTGATTTCGATACACAATTATTGCCTGATCGC
TTTACCCTACCTTTAGCTGCGCTTGGTTTAGGCATTAACACCTTTAATATCTACACCTCACCCAACTCGGCCATTTGGGG
TTACCTCATTGGTTTTCTATGTCTTTGGATTGTCTATTACTTATTTAAAGTAATTACTGGCAAAGAAGGTATGGGCTACG
GCGACTTTAAATTGCTTGCAGCATTAGGTGCATGGATGGGGCCATTGATGCTGCCGTTAATTGTGTTATTGTCATCGTTA
CTTGGCGCAATCATTGGCATCATTTTATTAAAATTAAGAAATGACAATCAGCCTTTTGCGTTTGGGCCATATATTGCCAT
TGCAGGTTGGGTTGCCTTTTTATGGGGTGATCAGATTATGAAAATTTATTTGGGAGGTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (138-247)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-128)

3D structure

• AlphaFold DB: A0A2L1VKF8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter nosocomialis M2	99.65	100	0.997
pilD	Acinetobacter baumannii D1279779	97.902	100	0.979
pilD	Vibrio cholerae strain A1552	53.676	95.105	0.51
pilD	Vibrio campbellii strain DS40M4	52.03	94.755	0.493
pilD	Neisseria gonorrhoeae MS11	47.727	92.308	0.441