Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   B7D75_RS23415 Genome accession   NZ_CP020560
Coordinates   4941166..4942758 (+) Length   530 a.a.
NCBI ID   WP_043101193.1    Uniprot ID   -
Organism   Pseudomonas paraeruginosa strain Cr1     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 4936166..4947758
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B7D75_RS23395 (B7D75_23410) pgeF 4937919..4938647 (-) 729 WP_043101190.1 peptidoglycan editing factor PgeF -
  B7D75_RS23400 (B7D75_23415) rluD 4938644..4939606 (-) 963 WP_003150035.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  B7D75_RS23405 (B7D75_23420) - 4939752..4940777 (+) 1026 WP_012077305.1 outer membrane protein assembly factor BamD -
  B7D75_RS23410 (B7D75_23425) - 4940931..4941176 (+) 246 WP_003150030.1 PP0621 family protein -
  B7D75_RS23415 (B7D75_23430) pilS 4941166..4942758 (+) 1593 WP_043101193.1 two-component system sensor histidine kinase PilS Regulator
  B7D75_RS23420 (B7D75_23435) pilR 4942774..4944111 (+) 1338 WP_033999343.1 two-component system response regulator PilR Regulator
  B7D75_RS23425 (B7D75_23440) thiO 4944157..4945251 (-) 1095 WP_041025522.1 glycine oxidase ThiO -
  B7D75_RS23430 (B7D75_23445) - 4945386..4945895 (+) 510 WP_003150026.1 GspH/FimT family pseudopilin -
  B7D75_RS23435 (B7D75_23450) - 4946001..4946507 (+) 507 WP_012077308.1 Tfp pilus assembly protein FimT/FimU -
  B7D75_RS23440 (B7D75_23455) pilV 4946498..4947055 (+) 558 WP_053814569.1 type IV pilus modification protein PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59197.44 Da        Isoelectric Point: 7.1538

>NTDB_id=224132 B7D75_RS23415 WP_043101193.1 4941166..4942758(+) (pilS) [Pseudomonas paraeruginosa strain Cr1]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEEQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLLLCGLFYAGGGMPSGIGSLLVVAVAIANILLRGRIGLVIAAVASLGLLYLTFFLSLSSPDAPNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRLNILLANQAALGLLRQDQVRGTSLD
RYSPMLVHCMKQWRLNPSRRPPTLKVIPEGPAVQPSFISLNREHDRHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDGADRRLTQIIQDQSRRMNLVIENVLQLSRRRQAEPQQLDLKDWLQRFVDEYPGKLRND
SELHLQLGAGDIHTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDADSDLPVLEIADDGPGVPTDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=224132 B7D75_RS23415 WP_043101193.1 4941166..4942758(+) (pilS) [Pseudomonas paraeruginosa strain Cr1]
GTGCGCGCTGAGCGGCTGCGGCTGAGCGAGGAGCAAGGGCAGCGCATCCTCCGCCTGTATCACCTGTATCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAGCTGGAAGAACAGGTCCTGAAACTGGTCCATCCCGAACTGTTCCATG
TCGGCAGCTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCACGGCAATTGCTGCCGATTTTC
ATCCTCGCCCTCACCGACGTGCTGCTGCTCTGCGGCCTGTTCTACGCCGGCGGCGGCATGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCGATCGCCAACATCCTCCTGCGCGGGCGCATCGGCCTGGTGATCGCGGCGGTGGCCAGCCTGG
GCCTGCTGTACCTGACCTTCTTCCTCAGCCTGAGCAGCCCGGACGCCCCCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTGCGGCGCCAGGAACAGACGGAAACGCTGGCCGAGGAACG
CGCCGAGACGGTCGCCAACCTCGAAGAGCTCAACGCGCTGATCCTGCAGCGCATGCGCACCGGCATCCTGGTGGTCGACA
GCCGCCTGAACATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACCAGGTGCGGGGCACCAGCCTGGAT
CGCTACAGCCCGATGCTGGTGCACTGCATGAAGCAGTGGCGCCTCAACCCCAGTCGCCGCCCGCCGACCCTCAAGGTGAT
TCCGGAGGGCCCCGCCGTGCAGCCCAGCTTCATCAGCCTCAACCGTGAACACGACCGGCATGTGCTGATCTTCCTCGAAG
ACATCTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTCGCCGGCCTCGGCCGGCTGACCGCCGGCATCGCCCACGAG
ATCCGCAACCCGCTGGGCGCCATCAGTCACGCCGCGCAACTGCTGCAGGAGTCCGAGGAACTCGACGGCGCCGACCGCCG
CCTGACACAGATCATCCAGGACCAGTCGCGACGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGCCGCCGCC
AGGCCGAACCGCAGCAACTCGACCTGAAGGACTGGCTGCAACGCTTCGTCGACGAATATCCCGGCAAGCTGCGCAACGAC
AGCGAACTGCACCTGCAGCTCGGCGCCGGCGACATCCACACGCGCATGGACCCGCACCAGTTGAATCAGGTACTGAGCAA
CCTGGTGCAGAACGGCCTGCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGTCTCGCGCGCGATGCGG
ACAGCGACCTGCCGGTGCTGGAAATCGCCGACGACGGCCCCGGCGTGCCGACGGACAAGCTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAATTGTGCGAGAGCAACCAGGCGCGCAT
CGACTACCGCAATCGCGAGGAGGGTGGAGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

94.528

100

0.945

Multiple sequence alignment