Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   NMB_RS00265 Genome accession   NC_003112
Coordinates   55466..56692 (-) Length   408 a.a.
NCBI ID   WP_002218483.1    Uniprot ID   Q9K1N4
Organism   Neisseria meningitidis MC58     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 50466..61692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB_RS00260 (NMB0050) yccS 53030..55180 (-) 2151 WP_002221798.1 YccS family putative transporter -
  NMB_RS00265 (NMB0051) pilU 55466..56692 (-) 1227 WP_002218483.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  NMB_RS00270 (NMB0052) pilT 56855..57898 (-) 1044 WP_002218487.1 type IV pilus twitching motility protein PilT Machinery gene
  NMB_RS00275 (NMB0053) - 58018..58704 (+) 687 WP_002243931.1 YggS family pyridoxal phosphate-dependent enzyme -
  NMB_RS00280 (NMB0054) - 58704..59111 (+) 408 WP_002243932.1 hypothetical protein -
  NMB_RS00285 (NMB0055) proC 59142..59951 (+) 810 WP_225905354.1 pyrroline-5-carboxylate reductase -
  NMB_RS00290 (NMB0056) dksA 60098..60514 (+) 417 WP_002215267.1 RNA polymerase-binding protein DksA -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45674.28 Da        Isoelectric Point: 6.9640

>NTDB_id=22328 NMB_RS00265 WP_002218483.1 55466..56692(-) (pilU) [Neisseria meningitidis MC58]
MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=22328 NMB_RS00265 WP_002218483.1 55466..56692(-) (pilU) [Neisseria meningitidis MC58]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAATGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCGCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAGTCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACCACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCAGGAATTGGGCCTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCAGCCATATCGTTACCATCGAAGACCCGATTGAATTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCCGACGTGGTCTGCATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATCGACATCGCCC
TCAACCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAACAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGATCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(49-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9K1N4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

44.598

88.48

0.395
  pilU Pseudomonas stutzeri DSM 10701

44.663

87.255

0.39
  pilU Vibrio cholerae strain A1552

46.429

82.353

0.382