Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C2H83_RS16430 Genome accession   NZ_CP026028
Coordinates   3167851..3168510 (+) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain CIT     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3162851..3173510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2H83_RS16400 ygiS 3164134..3165741 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  C2H83_RS16410 mqsA 3165874..3166269 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  C2H83_RS16415 mqsR 3166271..3166567 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  C2H83_RS16420 ygiV 3166772..3167254 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  C2H83_RS16425 ygiW 3167307..3167699 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  C2H83_RS16430 ciaR 3167851..3168510 (+) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  C2H83_RS16435 qseC 3168507..3169856 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  C2H83_RS16440 ygiZ 3169902..3170234 (-) 333 WP_000917684.1 DUF2645 family protein -
  C2H83_RS16450 mdaB 3170553..3171134 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  C2H83_RS16455 ygiN 3171165..3171479 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  C2H83_RS16460 parE 3171527..3173419 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=221952 C2H83_RS16430 WP_001221495.1 3167851..3168510(+) (ciaR) [Escherichia coli strain CIT]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=221952 C2H83_RS16430 WP_001221495.1 3167851..3168510(+) (ciaR) [Escherichia coli strain CIT]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361

Multiple sequence alignment