Detailed information NTG0221946

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: C2H83_RS14955
• Genome accession: NZ_CP026028
• Coordinates: 2864582..2865574 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081588.1
• Uniprot ID: Q540P0
• Organism: Escherichia coli strain CIT
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 2859582..2870574

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C2H83_RS14935	otnK	2860358..2861524 (+)	1167	WP_001393459.1	3-oxo-tetronate kinase	-
C2H83_RS14940	ygbL	2861616..2862254 (+)	639	WP_001278994.1	aldolase	-
C2H83_RS14945	ygbM	2862259..2863035 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
C2H83_RS14950	ygbN	2863124..2864488 (+)	1365	WP_000104441.1	GntP family transporter	-
C2H83_RS14955	rpoS	2864582..2865574 (-)	993	WP_000081588.1	RNA polymerase sigma factor RpoS	Regulator
C2H83_RS14960	nlpD	2865637..2866731 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
C2H83_RS14965	pcm	2866916..2867542 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
C2H83_RS14970	surE	2867536..2868297 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
C2H83_RS14975	truD	2868278..2869327 (-)	1050	WP_000568943.1	tRNA pseudouridine(13) synthase TruD	-
C2H83_RS14980	ispF	2869324..2869803 (-)	480	WP_001219242.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
C2H83_RS14985	ispD	2869803..2870513 (-)	711	WP_000246138.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37971.86 Da
• Isoelectric Point: 4.5886

>NTDB_id=221946 C2H83_RS14955 WP_000081588.1 2864582..2865574(-) (rpoS) [Escherichia coli strain CIT]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEQEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=221946 C2H83_RS14955 WP_000081588.1 2864582..2865574(-) (rpoS) [Escherichia coli strain CIT]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAACAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

• Sigma-70 region 3 (Sigma70_r3) (174-248)

3D structure

• AlphaFold DB: Q540P0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715