Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CXW53_RS15340 Genome accession   NZ_CP025920
Coordinates   3100013..3100672 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 103605     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3095013..3105672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXW53_RS15315 parE 3095104..3096996 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  CXW53_RS15320 ygiN 3097044..3097358 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  CXW53_RS15325 mdaB 3097389..3097970 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CXW53_RS15330 ygiZ 3098289..3098621 (+) 333 WP_000912120.1 DUF2645 family protein -
  CXW53_RS15335 qseC 3098667..3100016 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  CXW53_RS15340 ciaR 3100013..3100672 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  CXW53_RS15345 ygiW 3100824..3101216 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CXW53_RS15350 ygiV 3101269..3101751 (+) 483 WP_000183494.1 AraC family transcriptional regulator -
  CXW53_RS15355 parC 3102297..3104555 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  CXW53_RS15360 plsC 3104788..3105525 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=221247 CXW53_RS15340 WP_001221502.1 3100013..3100672(-) (ciaR) [Escherichia coli strain 103605]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=221247 CXW53_RS15340 WP_001221502.1 3100013..3100672(-) (ciaR) [Escherichia coli strain 103605]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365