Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CXW72_RS02065 Genome accession   NZ_CP025913
Coordinates   420475..421134 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 203740     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 415475..426134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXW72_RS02030 ygiS 415900..417507 (-) 1608 WP_001735743.1 ABC transporter substrate-binding protein -
  CXW72_RS02035 mqsA 417640..418035 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  CXW72_RS02040 mqsR 418037..418213 (-) 177 Protein_386 type II toxin-antitoxin system toxin MqsR -
  CXW72_RS02045 - 418350..419047 (+) 698 WP_099550739.1 IS1 family transposase -
  CXW72_RS02050 mqsR 419063..419191 (-) 129 Protein_388 type II toxin-antitoxin system toxin MqsR -
  CXW72_RS02055 ygiV 419396..419878 (-) 483 Protein_389 AraC family transcriptional regulator -
  CXW72_RS02060 ygiW 419931..420323 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CXW72_RS02065 ciaR 420475..421134 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  CXW72_RS02070 qseC 421131..422480 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  CXW72_RS02075 ygiZ 422526..422858 (-) 333 WP_000917684.1 DUF2645 family protein -
  CXW72_RS02080 mdaB 423177..423758 (+) 582 WP_000072421.1 NADPH:quinone oxidoreductase MdaB -
  CXW72_RS02085 ygiN 423789..424103 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  CXW72_RS02090 parE 424151..426043 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=221043 CXW72_RS02065 WP_001221493.1 420475..421134(+) (ciaR) [Escherichia coli strain 203740]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=221043 CXW72_RS02065 WP_001221493.1 420475..421134(+) (ciaR) [Escherichia coli strain 203740]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365