Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CXW77_RS09395 Genome accession   NZ_CP025910
Coordinates   1925198..1925857 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 204446     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1920198..1930857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXW77_RS09370 parE 1920289..1922181 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  CXW77_RS09375 ygiN 1922229..1922543 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  CXW77_RS09380 mdaB 1922574..1923155 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CXW77_RS09385 ygiZ 1923474..1923806 (+) 333 WP_000912120.1 DUF2645 family protein -
  CXW77_RS09390 qseC 1923852..1925201 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  CXW77_RS09395 ciaR 1925198..1925857 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  CXW77_RS09400 ygiW 1926009..1926401 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CXW77_RS09405 ygiV 1926454..1926936 (+) 483 WP_000183494.1 AraC family transcriptional regulator -
  CXW77_RS09410 parC 1927482..1929740 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  CXW77_RS09415 plsC 1929973..1930710 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=220986 CXW77_RS09395 WP_001221502.1 1925198..1925857(-) (ciaR) [Escherichia coli strain 204446]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=220986 CXW77_RS09395 WP_001221502.1 1925198..1925857(-) (ciaR) [Escherichia coli strain 204446]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365