Detailed information NTG0220415

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: B4W66_RS01360
• Genome accession: NZ_CP020027
• Coordinates: 258660..259730 (+)
• Length: 356 a.a.
• NCBI ID: WP_010921893.1
• Uniprot ID: A0A4U9C068
• Organism: Streptococcus pyogenes strain STAB090229
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 253660..264730

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B4W66_RS01345 (B4W66_01350)	amiA	254188..256158 (+)	1971	WP_038433589.1	peptide ABC transporter substrate-binding protein	Regulator
B4W66_RS01350 (B4W66_01355)	amiC	256223..257725 (+)	1503	WP_011017334.1	ABC transporter permease	Regulator
B4W66_RS01355 (B4W66_01360)	amiD	257725..258651 (+)	927	WP_002986004.1	oligopeptide ABC transporter permease OppC	Regulator
B4W66_RS01360 (B4W66_01365)	amiE	258660..259730 (+)	1071	WP_010921893.1	ABC transporter ATP-binding protein	Regulator
B4W66_RS01365 (B4W66_01370)	amiF	259723..260646 (+)	924	WP_002986000.1	ATP-binding cassette domain-containing protein	Regulator
B4W66_RS09880 (B4W66_01375)	-	260684..260767 (-)	84	Protein_231	IS3 family transposase	-
B4W66_RS09625 (B4W66_01380)	-	260794..260973 (-)	180	WP_111695866.1	transposase	-
B4W66_RS01385	-	261269..262419 (-)	1151	Protein_233	IS3 family transposase	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39703.77 Da
• Isoelectric Point: 5.6707

>NTDB_id=220415 B4W66_RS01360 WP_010921893.1 258660..259730(+) (amiE) [Streptococcus pyogenes strain STAB090229]
MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFVGESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQEL
TDLKTNKEWAKIRGSKIATIFQDPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITHDLGVVASIADKVAVMYAGEIVE
FGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAIPGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAK
TWLLHPEAPKVQKPEVIQDLHQKILRKMSQQEEGNV

Nucleotide

• Length: 1071 bp

>NTDB_id=220415 B4W66_RS01360 WP_010921893.1 258660..259730(+) (amiE) [Streptococcus pyogenes strain STAB090229]
ATGACAAAAGAAAATAATGTAATCTTAACTGCCAAAGATGTGGTGGTAGAATTTGATGTGCGTGATCGTGTTTTAACAGC
TATCCGTAACGTCTCACTGGAACTTGTTGAAGGAGAAGTCCTTGCTTTTGTAGGGGAATCAGGCTCAGGTAAATCTGTTT
TAACAAAGACCTTTACAGGGATGTTGGAGTCTAATGGACGCATTGCTAATGGTTCAATTGTCTATCGTGGGCAAGAATTG
ACAGATTTAAAAACAAATAAAGAGTGGGCAAAGATTCGCGGCTCAAAAATCGCAACGATTTTCCAAGACCCAATGACCAG
TCTTAGTCCCATTAAAACTATTGGTAGCCAAATCACAGAAGTGATTATTAAGCACCAAAAAGTAAGTCATGCCAAAGCTA
AAGAAATGGCCCTTGATTACATGAATAAAGTGGGTATCCCAAATGCCAAAAAACGCTTTGAAGATTACCCATTTGAGTAT
TCAGGAGGAATGCGTCAACGTATTGTTATCGCTATTGCTTTAGCTTGTCGCCCAGATATTCTTATCTGTGATGAGCCAAC
AACAGCCCTTGATGTGACTATTCAAGCTCAAATCGTTGAGTTATTGAAGTCGCTTCAACGAGAATATCATTTCACCATTA
TCTTTATTACGCACGATTTAGGTGTTGTGGCAAGCATTGCAGATAAAGTGGCTGTCATGTATGCAGGAGAAATTGTTGAA
TTTGGAACAGTCGAAGAGATTTTCTATGATCCAAGACACCCCTATACATGGAGTTTGCTGTCTAGCTTACCGCAGTTGGC
AGATGAATCTGGTGAACTTTACGCTATTCCAGGAACGCCTCCATCACTTTATTCACCAATTATCGGAGATGCCTTTGCAC
TTCGCTCAGAATATGCTATGGTTTTAGACTTTGAAAAAGCACCTCCGGCGATTAACGTATCTGAGACTCATTGGGCCAAA
ACATGGCTTTTACACCCAGAGGCTCCAAAAGTTCAAAAACCAGAAGTCATTCAAGATTTGCATCAAAAAATCTTAAGGAA
AATGTCACAACAGGAGGAAGGAAATGTCTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-299)

3D structure

• AlphaFold DB: A0A4U9C068

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	76.667	100	0.775
amiE	Streptococcus thermophilus LMG 18311	75.556	100	0.764
amiE	Streptococcus thermophilus LMD-9	75.556	100	0.764
oppD	Streptococcus mutans UA159	56.677	94.663	0.537