Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiF   Type   Regulator
Locus tag   B4W66_RS01365 Genome accession   NZ_CP020027
Coordinates   259723..260646 (+) Length   307 a.a.
NCBI ID   WP_002986000.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain STAB090229     
Function   internalize XIP (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IScluster/Tn 261269..262419 259723..260646 flank 623


Gene organization within MGE regions


Location: 259723..262419
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B4W66_RS01365 (B4W66_01370) amiF 259723..260646 (+) 924 WP_002986000.1 ATP-binding cassette domain-containing protein Regulator
  B4W66_RS09880 (B4W66_01375) - 260684..260767 (-) 84 Protein_231 IS3 family transposase -
  B4W66_RS09625 (B4W66_01380) - 260794..260973 (-) 180 WP_111695866.1 transposase -
  B4W66_RS01385 - 261269..262419 (-) 1151 Protein_233 IS3 family transposase -

Sequence

Protein


Download         Length: 307 a.a.        Molecular weight: 34715.90 Da        Isoelectric Point: 6.5113

>NTDB_id=220416 B4W66_RS01365 WP_002986000.1 259723..260646(+) (amiF) [Streptococcus pyogenes strain STAB090229]
MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGESGSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKS
EANELIRKIQMIFQDPAASLNERATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFISDRIAVIHKGVIVEVAETEELFN
NPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHDYTLDKPSMVEIKPNHFVWANQAEIEKYQKEL

Nucleotide


Download         Length: 924 bp        

>NTDB_id=220416 B4W66_RS01365 WP_002986000.1 259723..260646(+) (amiF) [Streptococcus pyogenes strain STAB090229]
ATGTCTGAGAAATTAGTCGAAGTAAAAGACCTAGAAATTTCCTTCGGAGAAGGAAAGAAAAAATTTGTTGCAGTTAAAAA
TGCTAATTTCTTTATTAAAAAAGGAGAAACCTTTTCTTTAGTTGGAGAATCTGGGAGTGGTAAAACAACAATTGGTCGTG
CTATTATTGGTTTGAACGATACTAGTTCAGGTCAAATTTTATACGATGGGAAAGTAATTAATGGCAGAAAATCAAAATCA
GAAGCCAATGAGCTCATTCGTAAAATTCAAATGATTTTCCAAGATCCCGCTGCTAGTTTGAATGAACGAGCAACCGTTGA
CTACATCATTTCAGAAGGTCTTTATAACTTTAATCTGTTTAAAACAGAGGAAGAACGTAAAGAAAAAATTAAGAACATGA
TGGCCGAAGTTGGTTTGCTATCAGAGCATTTGACGCGCTACCCACATGAATTTTCAGGAGGTCAACGTCAGCGGATCGGT
ATCGCTAGAGCCCTAGTAATGAACCCTGAATTCGTTATTGCTGATGAACCGATTTCAGCTTTGGACGTTTCCGTTCGCGC
ACAGGTTTTAAACCTTCTCAAACGGATGCAAGCCGAAAAAGGTTTGACTTATCTCTTCATTGCCCATGATCTTTCAGTCG
TTCGCTTTATTTCAGATCGTATTGCGGTTATCCATAAAGGGGTTATTGTAGAAGTTGCAGAAACAGAAGAACTGTTTAAT
AACCCAATTCATCCCTACACCCAATCCTTGTTATCAGCCGTGCCTATCCCAGATCCAATTTTAGAACGTCAAAAAGAACT
TGTTGTCTATCATCCAGACCAGCATGATTATACATTAGATAAGCCATCGATGGTTGAAATCAAACCAAATCACTTTGTTT
GGGCAAACCAAGCAGAAATTGAAAAATATCAAAAAGAATTGTAA

Domains

Predicted by InterproScan.

ABC transporter (ABC_tran)

(25-178)

Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY)

(230-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiF Streptococcus thermophilus LMG 18311

82.353

99.674

0.821
  amiF Streptococcus thermophilus LMD-9

82.026

99.674

0.818
  amiF Streptococcus salivarius strain HSISS4

82.026

99.674

0.818

Multiple sequence alignment