Detailed information NTG0220314

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BS21228_RS03515
• Genome accession: NZ_CP020023
• Coordinates: 615705..616322 (-)
• Length: 205 a.a.
• NCBI ID: WP_014480312.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain ATCC 21228
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 610705..621322

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BS21228_RS03490 (BS21228_03455)	rpsT	611007..611273 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
BS21228_RS03495 (BS21228_03460)	holA	611288..612331 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
BS21228_RS22695	-	612371..612520 (-)	150	WP_003229985.1	hypothetical protein	-
BS21228_RS03500 (BS21228_03465)	yqzM	612561..612695 (+)	135	WP_003229983.1	YqzM family protein	-
BS21228_RS03505 (BS21228_03470)	comEC	612735..615065 (-)	2331	WP_033881047.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BS21228_RS03510 (BS21228_03475)	comEB	615069..615638 (-)	570	WP_003229978.1	ComE operon protein 2	-
BS21228_RS03515 (BS21228_03480)	comEA	615705..616322 (-)	618	WP_014480312.1	competence protein ComEA	Machinery gene
BS21228_RS03520 (BS21228_03485)	comER	616406..617227 (+)	822	WP_014480313.1	late competence protein ComER	-
BS21228_RS03525 (BS21228_03490)	yqeM	617293..618036 (-)	744	WP_014480314.1	class I SAM-dependent methyltransferase	-
BS21228_RS03530 (BS21228_03495)	rsfS	618033..618389 (-)	357	WP_014480315.1	ribosome silencing factor	-
BS21228_RS03535 (BS21228_03500)	yqeK	618407..618967 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BS21228_RS03540 (BS21228_03505)	nadD	618957..619526 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
BS21228_RS03545 (BS21228_03510)	yhbY	619538..619828 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
BS21228_RS03550 (BS21228_03515)	aroE	619822..620664 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21781.51 Da
• Isoelectric Point: 4.7220

>NTDB_id=220314 BS21228_RS03515 WP_014480312.1 615705..616322(-) (comEA) [Bacillus subtilis strain ATCC 21228]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=220314 BS21228_RS03515 WP_014480312.1 615705..616322(-) (comEA) [Bacillus subtilis strain ATCC 21228]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	99.512	100	0.995
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39