Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CXX14_RS10350 Genome accession   NZ_CP025849
Coordinates   2171548..2172207 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 600468     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2166548..2177207
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CXX14_RS10325 parE 2166639..2168531 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  CXX14_RS10330 ygiN 2168579..2168893 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  CXX14_RS10335 mdaB 2168924..2169505 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CXX14_RS10340 ygiZ 2169824..2170156 (+) 333 WP_000912120.1 DUF2645 family protein -
  CXX14_RS10345 qseC 2170202..2171551 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  CXX14_RS10350 ciaR 2171548..2172207 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  CXX14_RS10355 ygiW 2172359..2172751 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CXX14_RS10360 ygiV 2172804..2173286 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  CXX14_RS10365 parC 2173832..2176090 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  CXX14_RS10370 plsC 2176324..2177061 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=220192 CXX14_RS10350 WP_001221502.1 2171548..2172207(-) (ciaR) [Escherichia coli strain 600468]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=220192 CXX14_RS10350 WP_001221502.1 2171548..2172207(-) (ciaR) [Escherichia coli strain 600468]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365