Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   BVZ63_RS02085 Genome accession   NZ_CP020014
Coordinates   436559..437098 (+) Length   179 a.a.
NCBI ID   WP_044345365.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 11P6H     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 431559..442098
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVZ63_RS02065 (BVZ63_429) - 432016..432936 (-) 921 WP_005630123.1 ATP-binding cassette domain-containing protein -
  BVZ63_RS02070 (BVZ63_430) - 432936..433817 (-) 882 WP_005656358.1 metal ABC transporter substrate-binding protein -
  BVZ63_RS02075 (BVZ63_431) - 434182..435060 (-) 879 WP_005656360.1 D-alanyl-D-alanine carboxypeptidase family protein -
  BVZ63_RS02080 (BVZ63_433) - 435307..436458 (+) 1152 WP_044345367.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  BVZ63_RS02085 (BVZ63_434) pilF/pilF2 436559..437098 (+) 540 WP_044345365.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BVZ63_RS02090 (BVZ63_435) - 437170..438081 (+) 912 WP_005686812.1 helix-turn-helix domain-containing protein -
  BVZ63_RS02095 (BVZ63_436) ispG 438090..439196 (+) 1107 WP_005686814.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BVZ63_RS02100 (BVZ63_437) hisS 439206..440477 (+) 1272 WP_005686816.1 histidine--tRNA ligase -
  BVZ63_RS02105 (BVZ63_438) - 440495..441109 (+) 615 WP_005686818.1 YfgM family protein -
  BVZ63_RS02110 (BVZ63_439) iscX 441160..441354 (-) 195 WP_005626949.1 Fe-S cluster assembly protein IscX -
  BVZ63_RS02115 (BVZ63_440) fdx 441354..441695 (-) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20652.47 Da        Isoelectric Point: 9.2047

>NTDB_id=220044 BVZ63_RS02085 WP_044345365.1 436559..437098(+) (pilF/pilF2) [Haemophilus influenzae strain 11P6H]
MQTISKQLSAVIFPFIFSACVSQSVSNLDHQAAAKVRVELALSYLQQNNPQLAKINLDKALLHDKNYYLVHSALAHYYQQ
QGQIKNAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQTDTFENIALCAYSAKKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=220044 BVZ63_RS02085 WP_044345365.1 436559..437098(+) (pilF/pilF2) [Haemophilus influenzae strain 11P6H]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCTGTATCTAA
CTTAGATCATCAAGCTGCCGCCAAAGTGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCTACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAAAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAGCAAGCTCAACAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAACAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

93.296

100

0.933
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment