Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   BH100B_RS17430 Genome accession   NZ_CP025251
Coordinates   3444159..3444818 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain BH100 substr. MG2017     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3439159..3449818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BH100B_RS17415 (BH100B_03416) ygiS 3441364..3442971 (-) 1608 WP_001298384.1 ABC transporter substrate-binding protein -
  BH100B_RS17420 (BH100B_03417) ygiV 3443080..3443562 (-) 483 WP_000183491.1 GyrI-like domain-containing protein -
  BH100B_RS17425 (BH100B_03418) ygiW 3443615..3444007 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  BH100B_RS17430 (BH100B_03419) ciaR 3444159..3444818 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  BH100B_RS17435 (BH100B_03420) qseC 3444815..3446164 (+) 1350 WP_000673389.1 quorum sensing histidine kinase QseC -
  BH100B_RS17440 (BH100B_03421) ygiZ 3446210..3446542 (-) 333 WP_000914696.1 DUF2645 family protein -
  BH100B_RS27765 (BH100B_03422) - 3446549..3447259 (-) 711 WP_001588762.1 hypothetical protein -
  BH100B_RS27770 - 3447262..3447741 (-) 480 WP_000065328.1 Hcp family type VI secretion system effector -
  BH100B_RS17450 (BH100B_03423) mdaB 3447991..3448572 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  BH100B_RS17455 (BH100B_03424) ygiN 3448603..3448917 (+) 315 WP_000633738.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=217819 BH100B_RS17430 WP_001221493.1 3444159..3444818(+) (ciaR) [Escherichia coli strain BH100 substr. MG2017]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=217819 BH100B_RS17430 WP_001221493.1 3444159..3444818(+) (ciaR) [Escherichia coli strain BH100 substr. MG2017]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365