Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CWI22_RS27905 Genome accession   NZ_CP025053
Coordinates   5835955..5836578 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain PB354     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5830955..5841578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI22_RS27885 (CWI22_27885) aroB 5831550..5832656 (-) 1107 WP_003114574.1 3-dehydroquinate synthase -
  CWI22_RS27890 (CWI22_27890) aroK 5832706..5833224 (-) 519 WP_003095833.1 shikimate kinase AroK -
  CWI22_RS27895 (CWI22_27895) pilQ 5833236..5835380 (-) 2145 WP_100882931.1 type 4a pilus secretin PilQ Machinery gene
  CWI22_RS27900 (CWI22_27900) comL/pilP 5835434..5835958 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  CWI22_RS27905 (CWI22_27905) pilO 5835955..5836578 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  CWI22_RS27910 (CWI22_27910) pilN 5836575..5837171 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  CWI22_RS27915 (CWI22_27915) pilM 5837171..5838236 (-) 1066 Protein_5394 type IV pilus assembly protein PilM -
  CWI22_RS27920 (CWI22_27920) - 5838421..5840889 (+) 2469 WP_100882932.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=217028 CWI22_RS27905 WP_003103268.1 5835955..5836578(-) (pilO) [Pseudomonas aeruginosa strain PB354]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=217028 CWI22_RS27905 WP_003103268.1 5835955..5836578(-) (pilO) [Pseudomonas aeruginosa strain PB354]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAGCAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401