Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilX   Type   Machinery gene
Locus tag   NMB_RS04615 Genome accession   NC_003112
Coordinates   910223..910696 (+) Length   157 a.a.
NCBI ID   WP_002225356.1    Uniprot ID   -
Organism   Neisseria meningitidis MC58     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 908094..924318 910223..910696 within 0


Gene organization within MGE regions


Location: 908094..924318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB_RS04600 (NMB0887) pilV 908100..908708 (+) 609 WP_061706010.1 type IV pilus modification protein PilV Machinery gene
  NMB_RS04605 (NMB0888) pilJ 908705..909646 (+) 942 WP_002244084.1 PilW family protein Machinery gene
  NMB_RS04610 (NMB0889) pilK 909625..910218 (+) 594 WP_002244085.1 pilus assembly PilX family protein Machinery gene
  NMB_RS04615 (NMB0890) pilX 910223..910696 (+) 474 WP_002225356.1 PilX family type IV pilin Machinery gene
  NMB_RS04620 (NMB0892) - 912465..912833 (-) 369 Protein_886 AzlC family ABC transporter permease -
  NMB_RS04625 (NMB0893) dut 913033..913485 (+) 453 WP_002225353.1 dUTP diphosphatase -
  NMB_RS04630 (NMB0894) dapC 913563..914750 (+) 1188 WP_002225352.1 succinyldiaminopimelate transaminase -
  NMB_RS12150 - 914839..914964 (+) 126 Protein_889 IS5/IS1182 family transposase -
  NMB_RS04635 (NMB0895) yaaA 915013..915792 (+) 780 WP_002225351.1 peroxide stress protein YaaA -
  NMB_RS04650 (NMB0896) - 916321..917393 (+) 1073 Protein_891 tyrosine-type recombinase/integrase -
  NMB_RS04655 (NMB0897) - 917506..917886 (+) 381 WP_002220683.1 type II toxin-antitoxin system PemK/MazF family toxin -
  NMB_RS04660 (NMB0899) - 918434..918790 (+) 357 WP_002223441.1 DUF4760 domain-containing protein -
  NMB_RS04665 (NMB0900) - 919659..920576 (+) 918 WP_002219437.1 KilA-N domain-containing protein -
  NMB_RS04670 (NMB0901) - 920634..920843 (-) 210 WP_002220687.1 hypothetical protein -
  NMB_RS04675 - 920840..920980 (-) 141 WP_002225349.1 hypothetical protein -
  NMB_RS04680 (NMB0902) - 920980..921171 (-) 192 WP_002219435.1 type ISP restriction/modification enzyme -
  NMB_RS04685 (NMB0903) - 921174..921467 (-) 294 WP_002220689.1 hypothetical protein -
  NMB_RS04690 (NMB0904) - 921464..921724 (-) 261 WP_002223443.1 NGO1622 family putative holin -
  NMB_RS04695 (NMB0905) - 921759..921974 (-) 216 WP_002219434.1 hypothetical protein -
  NMB_RS04700 (NMB0906) - 922044..922898 (-) 855 WP_002219433.1 YfdQ family protein -
  NMB_RS04705 (NMB0907) - 922923..923282 (-) 360 WP_002219432.1 hypothetical protein -
  NMB_RS04710 (NMB0908) - 923350..923550 (-) 201 WP_002219431.1 hypothetical protein -
  NMB_RS04715 (NMB0909) - 923794..924219 (-) 426 WP_002219430.1 hypothetical protein -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 17521.30 Da        Isoelectric Point: 9.4258

>NTDB_id=21601 NMB_RS04615 WP_002225356.1 910223..910696(+) (pilX) [Neisseria meningitidis MC58]
MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTEMVGINNISKQFILKNPLDDNQTIENKLEIFVSGYKM
NPKIAKKYSVSVKFVDKEKSRAYRLVGVPKAGTGYTLSVWMNSVGDGYKCRDAASAQAHLETLSSDVGCEAFSNRKK

Nucleotide


Download         Length: 474 bp        

>NTDB_id=21601 NMB_RS04615 WP_002225356.1 910223..910696(+) (pilX) [Neisseria meningitidis MC58]
ATGGAACAAAAAGGGTTTACATTGATTGAGATGATGATAGTCGTCGCGATACTCGGCATTATCAGCGTCATTGCCATACC
TTCTTATCAAAGTTATATTGAAAAAGGCTATCAGTCCCAGCTTTATACGGAGATGGTCGGTATCAACAATATTTCCAAAC
AGTTTATTTTGAAAAATCCCCTGGACGATAATCAGACCATCGAGAACAAACTGGAAATATTTGTCTCAGGCTATAAGATG
AATCCGAAAATTGCCAAAAAATATAGTGTTTCGGTAAAGTTTGTCGATAAGGAAAAATCAAGGGCATACAGGTTGGTCGG
CGTTCCGAAGGCGGGGACGGGTTATACTTTGTCGGTATGGATGAACAGCGTGGGCGACGGATACAAATGCCGTGATGCCG
CTTCTGCCCAAGCCCATTTGGAGACCTTGTCCTCAGATGTCGGCTGTGAAGCCTTCTCTAATCGTAAAAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-25)

Minor type IV pilin, PilX (Pilin_PilX)

(31-157)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilX Neisseria meningitidis 8013

92.994

100

0.93
  pilL Neisseria gonorrhoeae MS11

88.535

100

0.885

Multiple sequence alignment