Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilK   Type   Machinery gene
Locus tag   NMB_RS04610 Genome accession   NC_003112
Coordinates   909625..910218 (+) Length   197 a.a.
NCBI ID   WP_002244085.1    Uniprot ID   Q9JZV1
Organism   Neisseria meningitidis MC58     
Function   type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 908094..924318 909625..910218 within 0


Gene organization within MGE regions


Location: 908094..924318
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMB_RS04600 (NMB0887) pilV 908100..908708 (+) 609 WP_061706010.1 type IV pilus modification protein PilV Machinery gene
  NMB_RS04605 (NMB0888) pilJ 908705..909646 (+) 942 WP_002244084.1 PilW family protein Machinery gene
  NMB_RS04610 (NMB0889) pilK 909625..910218 (+) 594 WP_002244085.1 pilus assembly PilX family protein Machinery gene
  NMB_RS04615 (NMB0890) pilX 910223..910696 (+) 474 WP_002225356.1 PilX family type IV pilin Machinery gene
  NMB_RS04620 (NMB0892) - 912465..912833 (-) 369 Protein_886 AzlC family ABC transporter permease -
  NMB_RS04625 (NMB0893) dut 913033..913485 (+) 453 WP_002225353.1 dUTP diphosphatase -
  NMB_RS04630 (NMB0894) dapC 913563..914750 (+) 1188 WP_002225352.1 succinyldiaminopimelate transaminase -
  NMB_RS12150 - 914839..914964 (+) 126 Protein_889 IS5/IS1182 family transposase -
  NMB_RS04635 (NMB0895) yaaA 915013..915792 (+) 780 WP_002225351.1 peroxide stress protein YaaA -
  NMB_RS04650 (NMB0896) - 916321..917393 (+) 1073 Protein_891 tyrosine-type recombinase/integrase -
  NMB_RS04655 (NMB0897) - 917506..917886 (+) 381 WP_002220683.1 type II toxin-antitoxin system PemK/MazF family toxin -
  NMB_RS04660 (NMB0899) - 918434..918790 (+) 357 WP_002223441.1 DUF4760 domain-containing protein -
  NMB_RS04665 (NMB0900) - 919659..920576 (+) 918 WP_002219437.1 KilA-N domain-containing protein -
  NMB_RS04670 (NMB0901) - 920634..920843 (-) 210 WP_002220687.1 hypothetical protein -
  NMB_RS04675 - 920840..920980 (-) 141 WP_002225349.1 hypothetical protein -
  NMB_RS04680 (NMB0902) - 920980..921171 (-) 192 WP_002219435.1 type ISP restriction/modification enzyme -
  NMB_RS04685 (NMB0903) - 921174..921467 (-) 294 WP_002220689.1 hypothetical protein -
  NMB_RS04690 (NMB0904) - 921464..921724 (-) 261 WP_002223443.1 NGO1622 family putative holin -
  NMB_RS04695 (NMB0905) - 921759..921974 (-) 216 WP_002219434.1 hypothetical protein -
  NMB_RS04700 (NMB0906) - 922044..922898 (-) 855 WP_002219433.1 YfdQ family protein -
  NMB_RS04705 (NMB0907) - 922923..923282 (-) 360 WP_002219432.1 hypothetical protein -
  NMB_RS04710 (NMB0908) - 923350..923550 (-) 201 WP_002219431.1 hypothetical protein -
  NMB_RS04715 (NMB0909) - 923794..924219 (-) 426 WP_002219430.1 hypothetical protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 21699.65 Da        Isoelectric Point: 6.3911

>NTDB_id=21600 NMB_RS04610 WP_002244085.1 909625..910218(+) (pilK) [Neisseria meningitidis MC58]
MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNTEQRISANESDRKLALSLAEAALREGELQVLDLEYDT
DSKVTFSENCGKGLCAAVNVRTNNDNEEAFDNIVVQGKPTVEAVKRSCPANSTDLCIDKKGMEYKKGTRSVSKMPRYIIE
YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE

Nucleotide


Download         Length: 594 bp        

>NTDB_id=21600 NMB_RS04610 WP_002244085.1 909625..910218(+) (pilK) [Neisseria meningitidis MC58]
ATGCGCAAACAGAACACTTTGACGGGAATCCCGACTTCTGACGGACAGAGGGGGTTTGCACTGTTTATCGTGCTGATGGT
GATGATCGTCGTGGCTTTTTTGGTTGTAACTGCCGCGCAGTCTTACAATACCGAGCAGCGGATCAGTGCCAACGAATCAG
ACAGGAAATTGGCTTTGTCTTTGGCCGAGGCGGCTTTGCGGGAAGGCGAACTTCAGGTTTTGGATTTGGAATATGATACG
GACAGTAAGGTTACATTTAGCGAAAACTGTGGAAAAGGTCTGTGTGCCGCAGTGAATGTGCGGACAAATAATGATAATGA
AGAGGCTTTTGACAATATCGTGGTGCAAGGCAAGCCCACCGTTGAGGCGGTGAAGCGTTCTTGCCCTGCAAATTCTACCG
ACCTGTGCATTGACAAGAAAGGGATGGAATATAAGAAAGGCACGAGAAGCGTCAGCAAAATGCCACGTTATATTATCGAA
TATTTGGGCGTGAAGAACGGAGAAAATGTTTATCGGGTTACTGCCAAGGCTTGGGGTAAGAATGCCAATACCGTGGTCGT
CCTTCAATCTTATGTAAGCAATAATGATGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilX C-term (PilX)

(92-192)

PilX N-terminal (PilX_N)

(17-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9JZV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilK Neisseria gonorrhoeae MS11

86.634

100

0.888

Multiple sequence alignment