Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PspS35_RS01995 Genome accession   NZ_CP019431
Coordinates   467820..469145 (+) Length   441 a.a.
NCBI ID   WP_159937968.1    Uniprot ID   A0A6I6VQ32
Organism   Pseudomonas sp. S35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 462820..474145
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspS35_RS01975 (PspS35_02015) - 462898..465360 (-) 2463 WP_159932543.1 penicillin-binding protein 1A -
  PspS35_RS01980 (PspS35_02020) pilM 465579..466232 (+) 654 WP_174244852.1 type IV pilus assembly protein PilM -
  PspS35_RS01985 (PspS35_02025) - 466229..466783 (+) 555 WP_159932544.1 PilN domain-containing protein -
  PspS35_RS01990 (PspS35_02030) - 466780..467751 (+) 972 WP_159932545.1 pilus assembly protein PilP -
  PspS35_RS01995 (PspS35_02035) pilQ 467820..469145 (+) 1326 WP_159937968.1 type IV pilus secretin PilQ family protein Machinery gene
  PspS35_RS02000 (PspS35_02040) aroK 469150..469668 (+) 519 WP_159932546.1 shikimate kinase AroK -
  PspS35_RS02005 (PspS35_02045) aroB 469794..470894 (+) 1101 WP_159932547.1 3-dehydroquinate synthase -
  PspS35_RS02010 (PspS35_02050) - 470905..472461 (+) 1557 WP_159932548.1 AAA family ATPase -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 48115.12 Da        Isoelectric Point: 4.8234

>NTDB_id=215721 PspS35_RS01995 WP_159937968.1 467820..469145(+) (pilQ) [Pseudomonas sp. S35]
MAAALPNRMDLIPLQPPGNAVSKTYTGDTLNLNFQNIELRAVLQQIADVAGLNLVASDDVQGSITLRLKDVPWDQALDLV
LQTKGLDKRVRAGVLLVAPAEELAARELLNLESRKQMADLAPLRRELLQVNYAKAADLAKLFQSVSGLEGTTDERGSVAV
DDRTNNIIAYQTVERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGARWGGRLHRGNWSAGGINKPLPEGVEPPE
APPSSPFVDMGALTATSGLGIAYITDNLLLDLELNAMEKTGNGEIVSQPKVVTADKETARILKGTEIPYQESSSSGATSV
SFKEASLSLEVTPQITPDDRVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=215721 PspS35_RS01995 WP_159937968.1 467820..469145(+) (pilQ) [Pseudomonas sp. S35]
ATGGCTGCTGCCCTGCCCAATCGTATGGATCTGATCCCGTTACAACCCCCTGGTAATGCGGTTTCGAAGACTTACACCGG
CGACACGCTCAACCTCAACTTCCAGAACATCGAACTGCGGGCGGTATTGCAGCAGATCGCCGACGTAGCGGGCCTCAACC
TGGTGGCCAGCGACGATGTGCAAGGCTCCATCACGCTGCGGCTCAAGGACGTGCCCTGGGACCAGGCGCTGGACCTGGTC
CTGCAAACCAAGGGGCTGGATAAGCGCGTAAGGGCCGGGGTCTTGCTGGTGGCACCCGCGGAGGAATTGGCCGCGCGCGA
ACTGCTGAACCTGGAGTCGCGCAAGCAAATGGCCGATCTCGCACCGTTGCGCCGGGAATTGCTGCAGGTCAATTACGCCA
AGGCGGCGGACCTGGCCAAGCTGTTCCAGTCGGTGAGCGGCCTGGAAGGCACGACCGATGAGCGGGGCTCGGTGGCGGTG
GATGACCGCACCAATAATATCATTGCCTACCAGACCGTCGAACGGCTTGAAGAGCTGCGGCGAATCGTGGCACAACTGGA
TATCCCTGTGCGCCAAGTGATGATCGAGGCGCGTATTGTCGAGGCCAATGTCGACTACGACAAAAGCCTGGGAGCACGCT
GGGGAGGGCGGCTTCATCGCGGCAACTGGAGCGCTGGCGGTATCAACAAACCCTTGCCCGAAGGTGTGGAGCCGCCGGAA
GCTCCGCCGAGCTCGCCGTTTGTAGATATGGGGGCGCTCACCGCGACTTCGGGCCTGGGGATCGCCTACATCACCGATAA
CCTGCTGCTCGACCTGGAACTGAACGCCATGGAGAAAACCGGCAACGGTGAAATCGTCTCGCAGCCCAAAGTCGTCACCG
CCGACAAGGAGACCGCGCGCATCCTCAAGGGCACCGAGATCCCGTACCAGGAATCGAGCTCTAGCGGTGCCACCTCGGTT
TCGTTCAAGGAGGCTTCCCTATCGCTGGAAGTCACCCCGCAGATCACTCCCGACGACCGCGTGATCATGGAGGTGAAAGT
CACCAAGGATGAGCCCGACTACCTCAACAAACTCAACGACGTACCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGT
TGGTCAAGGATGGCGAGACCATCGTGATTGGCGGGGTTTTTTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCAGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(276-432)

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I6VQ32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.56

98.866

0.707
  comQ Acinetobacter baylyi ADP1

42.07

100

0.433
  pilQ Acinetobacter baumannii strain A118

40.821

100

0.429
  pilQ Acinetobacter baumannii D1279779

40.821

100

0.429
  pilQ Legionella pneumophila strain ERS1305867

44.181

95.465

0.422
  pilQ Legionella pneumophila strain Lp02

44.181

95.465

0.422
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.498

94.785

0.422
  pilQ Vibrio cholerae strain A1552

44.498

94.785

0.422
  pilQ Vibrio campbellii strain DS40M4

43.972

95.918

0.422

Multiple sequence alignment