Detailed information NTG0214959

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BST96_RS08765
• Genome accession: NZ_CP019343
• Coordinates: 1905877..1906944 (+)
• Length: 355 a.a.
• NCBI ID: WP_157117909.1
• Uniprot ID: A0A1X9NAX8
• Organism: Oceanicoccus sagamiensis strain NBRC 107125
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1900877..1911944

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BST96_RS08755 (BST96_08765)	radA	1901735..1903105 (+)	1371	WP_085758338.1	DNA repair protein RadA	Machinery gene
BST96_RS08760 (BST96_08770)	-	1903199..1905703 (-)	2505	WP_085758339.1	penicillin-binding protein 1A	-
BST96_RS08765 (BST96_08775)	pilM	1905877..1906944 (+)	1068	WP_157117909.1	pilus assembly protein PilM	Machinery gene
BST96_RS08770 (BST96_08780)	-	1906944..1907510 (+)	567	WP_085758341.1	PilN domain-containing protein	-
BST96_RS08775 (BST96_08785)	-	1907514..1908134 (+)	621	WP_085758342.1	type 4a pilus biogenesis protein PilO	-
BST96_RS08780 (BST96_08790)	-	1908124..1908660 (+)	537	WP_338043305.1	pilus assembly protein PilP	-
BST96_RS08785 (BST96_08795)	-	1908678..1910882 (+)	2205	WP_085758344.1	type IV pilus secretin PilQ	-
BST96_RS08790 (BST96_08800)	aroK	1910925..1911440 (+)	516	WP_085758345.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 355 a.a.
• Molecular weight: 38489.54 Da
• Isoelectric Point: 4.1209

>NTDB_id=214959 BST96_RS08765 WP_157117909.1 1905877..1906944(+) (pilM) [Oceanicoccus sagamiensis strain NBRC 107125]
MLGSLFGKKKSIPVLGVDISSTSVKLLEISKSGDQYKVESYAVASLPPNAVVEKNIAELEGVAESVTKAYERSKSKMKTA
AVAVAGSAVITKLIDMPEGLNDDNMETQITLEADQYIPYPLDEVAIDFEVQGESDNSPEQVEVLLAACRRENVELRVDAL
EMADITAKVVDVEAYAIERTFELIRPHLDCDEDSAIAIIDIGSTMTTLSVLVDGQTVYTREQLFGGRQLTEEIQRRYGLS
LDEAGLAKKQGGLPDDYEPEVLEPFKDAVVQQVTRSLQFFFSSSQYNDVDHIVLAGGVASMTGLAEVVQDKLGTPTTLAN
PFTNMSVASRVDTMSLSNDAPSLMIACGLALRSFD

Nucleotide

• Length: 1068 bp

>NTDB_id=214959 BST96_RS08765 WP_157117909.1 1905877..1906944(+) (pilM) [Oceanicoccus sagamiensis strain NBRC 107125]
GTGTTAGGCAGTCTATTCGGAAAAAAGAAATCGATCCCAGTGCTGGGGGTAGACATCAGTTCTACCTCGGTCAAGCTACT
GGAGATTAGTAAAAGTGGCGACCAATACAAGGTCGAGAGCTACGCTGTGGCCTCCTTGCCGCCCAATGCTGTGGTGGAGA
AAAACATCGCAGAGCTGGAAGGCGTCGCTGAATCTGTCACCAAGGCCTATGAGCGTTCCAAGTCGAAAATGAAGACTGCG
GCGGTCGCCGTTGCCGGTTCTGCGGTTATCACCAAGTTGATTGATATGCCCGAGGGCCTCAACGACGATAATATGGAAAC
CCAGATAACCCTTGAAGCTGACCAGTATATTCCCTACCCCCTGGATGAAGTCGCGATCGACTTTGAGGTTCAGGGTGAGT
CAGATAATAGTCCCGAGCAGGTGGAAGTCTTACTGGCCGCCTGCCGCCGTGAAAACGTCGAGCTGCGCGTTGATGCTTTG
GAGATGGCGGATATTACCGCGAAGGTCGTTGATGTTGAGGCCTATGCCATCGAGCGCACCTTTGAGCTGATCCGTCCTCA
TCTGGACTGCGATGAAGACAGTGCTATCGCTATTATTGATATAGGCTCTACCATGACCACCTTAAGTGTTCTGGTCGATG
GCCAAACCGTTTATACCCGTGAGCAGCTCTTTGGCGGACGCCAGCTCACCGAAGAAATTCAGCGCCGCTATGGCCTCTCT
TTGGATGAGGCTGGCCTTGCCAAGAAACAGGGCGGTTTGCCCGACGATTATGAGCCTGAAGTCCTTGAGCCATTTAAAGA
CGCCGTGGTTCAGCAAGTAACTCGCTCACTACAATTCTTCTTTTCTTCCAGTCAATACAATGATGTCGACCATATCGTAC
TGGCTGGCGGTGTCGCTTCAATGACCGGCCTGGCAGAAGTTGTTCAAGACAAGCTGGGCACACCCACCACCCTGGCGAAT
CCGTTTACCAATATGTCGGTGGCTTCCCGGGTCGATACCATGTCATTAAGTAACGATGCTCCTTCACTAATGATCGCCTG
CGGTTTGGCGCTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (16-354)

3D structure

• AlphaFold DB: A0A1X9NAX8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.609	98.028	0.555
comM	Acinetobacter nosocomialis M2	56.609	98.028	0.555
comM	Acinetobacter baylyi ADP1	53.509	96.338	0.515
pilM	Legionella pneumophila strain ERS1305867	47.191	100	0.473