Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BRL61_RS08280 Genome accession   NZ_CP019117
Coordinates   1652832..1653518 (-) Length   228 a.a.
NCBI ID   WP_001794049.1    Uniprot ID   A0A0E1X4U4
Organism   Staphylococcus aureus strain SJTUF_J27     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1647832..1658518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BRL61_RS08260 (BRL61_08255) rpsT 1648746..1648997 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  BRL61_RS08265 (BRL61_08260) holA 1649042..1650016 (-) 975 WP_001282570.1 DNA polymerase III subunit delta -
  BRL61_RS08270 (BRL61_08265) comEC 1650073..1652274 (-) 2202 WP_000967506.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BRL61_RS08275 (BRL61_08270) - 1652279..1652740 (-) 462 WP_000439692.1 ComE operon protein 2 -
  BRL61_RS08280 (BRL61_08275) comEA 1652832..1653518 (-) 687 WP_001794049.1 ComEA family DNA-binding protein Machinery gene
  BRL61_RS08285 (BRL61_08280) - 1653558..1654274 (-) 717 WP_070004469.1 class I SAM-dependent DNA methyltransferase -
  BRL61_RS08290 (BRL61_08285) rsfS 1654277..1654630 (-) 354 WP_001088020.1 ribosome silencing factor -
  BRL61_RS08295 (BRL61_08290) yqeK 1654631..1655215 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BRL61_RS08300 (BRL61_08295) nadD 1655205..1655774 (-) 570 WP_000822938.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  BRL61_RS08305 (BRL61_08300) yhbY 1655777..1656067 (-) 291 WP_000955232.1 ribosome assembly RNA-binding protein YhbY -
  BRL61_RS08310 (BRL61_08305) aroE 1656071..1656877 (-) 807 WP_000666750.1 shikimate dehydrogenase -
  BRL61_RS08315 (BRL61_08310) yqeH 1656891..1657991 (-) 1101 WP_053865719.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25766.38 Da        Isoelectric Point: 9.3383

>NTDB_id=213235 BRL61_RS08280 WP_001794049.1 1652832..1653518(-) (comEA) [Staphylococcus aureus strain SJTUF_J27]
MVLLYQFLLRYKDFLAQWKLYIISAVVLIMVLIGFIFWRQDDYTSRDFENKDTVLKQSTSENSSLSKLEDVQVKDGDNSK
NKGPVYVDIKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQFGANSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLNSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=213235 BRL61_RS08280 WP_001794049.1 1652832..1653518(-) (comEA) [Staphylococcus aureus strain SJTUF_J27]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAGCTCAATGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAGATTTTGAAAATAAAGATACTG
TTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATATAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTAGATGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATTCCTCATAAAGGACAAAAGAATGTTGAACCACAATTTGGAGCAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAATTCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E1X4U4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

95.595

99.561

0.952
  comEA Staphylococcus aureus N315

95.595

99.561

0.952

Multiple sequence alignment