Detailed information NTG0212765

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BVH55_RS13340
• Genome accession: NZ_CP019040
• Coordinates: 2660555..2661169 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain GH1-13
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2655555..2666169

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BVH55_RS13315 (BVH55_13345)	rpsT	2655855..2656121 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BVH55_RS13320 (BVH55_13350)	holA	2656138..2657179 (-)	1042	Protein_2506	DNA polymerase III subunit delta	-
BVH55_RS20865	-	2657219..2657373 (-)	155	Protein_2507	hypothetical protein	-
BVH55_RS13325 (BVH55_13355)	-	2657414..2657548 (+)	135	WP_003152870.1	YqzM family protein	-
BVH55_RS13330 (BVH55_13360)	comEC	2657567..2659918 (-)	2352	WP_077722611.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BVH55_RS13335 (BVH55_13365)	-	2659919..2660488 (-)	570	WP_003152868.1	ComE operon protein 2	-
BVH55_RS13340 (BVH55_13370)	comEA	2660555..2661169 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
BVH55_RS13345 (BVH55_13375)	comER	2661228..2662049 (+)	822	WP_014305449.1	late competence protein ComER	-
BVH55_RS13350 (BVH55_13380)	-	2662118..2662855 (-)	738	WP_031378935.1	class I SAM-dependent methyltransferase	-
BVH55_RS13355 (BVH55_13385)	rsfS	2662852..2663208 (-)	357	WP_003152864.1	ribosome silencing factor	-
BVH55_RS13360 (BVH55_13390)	yqeK	2663226..2663786 (-)	561	WP_077722612.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BVH55_RS13365 (BVH55_13395)	-	2663776..2664345 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
BVH55_RS13370 (BVH55_13400)	yhbY	2664356..2664646 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BVH55_RS13375 (BVH55_13405)	aroE	2664640..2665482 (-)	843	WP_015417858.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=212765 BVH55_RS13340 WP_013352942.1 2660555..2661169(-) (comEA) [Bacillus velezensis strain GH1-13]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=212765 BVH55_RS13340 WP_013352942.1 2660555..2661169(-) (comEA) [Bacillus velezensis strain GH1-13]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363