Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   A5960_RS24270 Genome accession   NZ_CP024289
Coordinates   4651195..4651854 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli O178:H19 strain 2012C-4431     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4646195..4656854
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A5960_RS24245 parE 4646286..4648178 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  A5960_RS24250 ygiN 4648226..4648540 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  A5960_RS24255 mdaB 4648571..4649152 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  A5960_RS24260 ygiZ 4649471..4649803 (+) 333 WP_000912120.1 DUF2645 family protein -
  A5960_RS24265 qseC 4649849..4651198 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  A5960_RS24270 ciaR 4651195..4651854 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  A5960_RS24275 ygiW 4652006..4652398 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  A5960_RS24280 ygiV 4652451..4652933 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  A5960_RS24290 parC 4653479..4655737 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  A5960_RS24295 plsC 4655970..4656707 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=212717 A5960_RS24270 WP_001221502.1 4651195..4651854(-) (ciaR) [Escherichia coli O178:H19 strain 2012C-4431]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=212717 A5960_RS24270 WP_001221502.1 4651195..4651854(-) (ciaR) [Escherichia coli O178:H19 strain 2012C-4431]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment