Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   SAR_RS08280 Genome accession   NC_002952
Coordinates   1688475..1688972 (-) Length   165 a.a.
NCBI ID   WP_001794055.1    Uniprot ID   A0A7U7EV69
Organism   Staphylococcus aureus subsp. aureus MRSA252     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1683475..1693972
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAR_RS08255 (SAR1612) gcvPB 1683637..1685109 (-) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  SAR_RS08260 (SAR1613) gcvPA 1685102..1686448 (-) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  SAR_RS08265 (SAR1614) gcvT 1686468..1687559 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  SAR_RS08270 (SAR1615) - 1687718..1688242 (-) 525 WP_001015125.1 shikimate kinase -
  SAR_RS08275 - 1688232..1688378 (-) 147 WP_001789879.1 hypothetical protein -
  SAR_RS08280 (SAR1616) comGF 1688475..1688972 (-) 498 WP_001794055.1 competence type IV pilus minor pilin ComGF Machinery gene
  SAR_RS08285 (SAR1617) comGE 1688890..1689189 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  SAR_RS08290 (SAR1618) comGD 1689176..1689622 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  SAR_RS08295 (SAR1619) comGC 1689600..1689911 (-) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  SAR_RS08300 (SAR1620) comGB 1689925..1690995 (-) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  SAR_RS08305 (SAR1621) comGA 1690967..1691941 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  SAR_RS08310 (SAR1622) - 1691993..1692616 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  SAR_RS08315 (SAR1623) - 1692613..1692942 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  SAR_RS08320 (SAR1624) - 1692942..1693928 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19224.98 Da        Isoelectric Point: 10.2192

>NTDB_id=21271 SAR_RS08280 WP_001794055.1 1688475..1688972(-) (comGF) [Staphylococcus aureus subsp. aureus MRSA252]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLNKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=21271 SAR_RS08280 WP_001794055.1 1688475..1688972(-) (comGF) [Staphylococcus aureus subsp. aureus MRSA252]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAACAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7EV69

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

96.97

100

0.97

Multiple sequence alignment