Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BVG12_RS22290 Genome accession   NZ_CP019038
Coordinates   4521005..4522504 (+) Length   499 a.a.
NCBI ID   WP_075794301.1    Uniprot ID   -
Organism   Massilia putida strain 6NM-7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4516005..4527504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVG12_RS22265 - 4517420..4518442 (+) 1023 WP_075794298.1 KpsF/GutQ family sugar-phosphate isomerase -
  BVG12_RS22270 - 4518442..4518975 (+) 534 WP_179966237.1 HAD family hydrolase -
  BVG12_RS22275 lptC 4518991..4519593 (+) 603 WP_075794299.1 LPS export ABC transporter periplasmic protein LptC -
  BVG12_RS22280 lptA 4519633..4520211 (+) 579 WP_075794300.1 lipopolysaccharide transport periplasmic protein LptA -
  BVG12_RS22285 lptB 4520208..4521002 (+) 795 WP_083685312.1 LPS export ABC transporter ATP-binding protein -
  BVG12_RS22290 rpoN/rpoN1 4521005..4522504 (+) 1500 WP_075794301.1 RNA polymerase factor sigma-54 Machinery gene
  BVG12_RS22295 hpf 4522575..4522934 (+) 360 WP_075794302.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BVG12_RS22300 - 4523162..4524247 (+) 1086 WP_075794303.1 enoyl-CoA hydratase/isomerase family protein -
  BVG12_RS22305 - 4524244..4524717 (+) 474 WP_075794304.1 hypothetical protein -
  BVG12_RS22310 - 4524714..4525494 (+) 781 Protein_3970 APH(3')-II family aminoglycoside O-phosphotransferase -
  BVG12_RS22315 rapZ 4525586..4526449 (-) 864 WP_075794306.1 RNase adapter RapZ -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 54678.10 Da        Isoelectric Point: 4.8732

>NTDB_id=212705 BVG12_RS22290 WP_075794301.1 4521005..4522504(+) (rpoN/rpoN1) [Massilia putida strain 6NM-7]
MKQTLQLKTSQHLALTPQLQQSIRLLQLSTLELHQEIEQVLSENPLLERLDDPLDRSVRLLADGAINSAPASTQAGEAPA
AQGSGEEGGAPAEHGNGENGEAGGDSGEGGGSEAGEDWGEYGRSGSGGDDEDGRPQLEASPVTLREHLMEQVRLTCCAPR
DCALVELVVDALDDNGYLEEPLEDIHARLPAELEIEFEELRMALALVQSMDPPGVGARSAAECLALQIRRMPGVPLVTRR
MALVIVEKYLTWFAQREFNKLKKALDCDDEDLREAQAVIRQCNPHPGAAFASDVSDFVVPDVIVRRAKDGWSVQLNPEVM
PRLRVNSLYASLMKQGKSETQLGAQLQEAKWLIKNMRQRFDTILRVAQAIVDRQRNFFSHGAVAMRPLVLREIADTLGLH
ESTISRVTTQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIAEMLGEQGMVIARRT
VAKYREALKIPPVSLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=212705 BVG12_RS22290 WP_075794301.1 4521005..4522504(+) (rpoN/rpoN1) [Massilia putida strain 6NM-7]
ATGAAACAGACGCTGCAACTCAAGACTTCACAGCACCTTGCACTGACGCCCCAACTCCAGCAATCGATCCGGCTGTTGCA
GCTGTCGACTCTGGAGCTGCACCAGGAAATCGAGCAGGTCTTGAGCGAGAACCCCTTGCTCGAACGGCTCGACGATCCGC
TCGACCGGTCGGTGCGCCTGCTGGCCGACGGCGCCATCAACAGCGCGCCGGCATCGACGCAGGCCGGTGAGGCGCCGGCC
GCGCAGGGCTCGGGCGAGGAGGGCGGCGCGCCCGCCGAGCATGGCAATGGCGAGAACGGCGAAGCGGGTGGCGACAGCGG
CGAAGGCGGCGGCAGCGAAGCCGGCGAGGACTGGGGCGAGTACGGCCGCAGCGGCAGCGGCGGCGACGATGAAGACGGCC
GCCCGCAACTGGAAGCGAGTCCCGTCACGCTGCGCGAGCACCTGATGGAGCAGGTGCGCCTGACGTGCTGCGCGCCGCGC
GACTGCGCGCTCGTGGAACTCGTCGTCGATGCGCTGGACGACAACGGTTATCTGGAAGAGCCGCTGGAAGACATCCACGC
GCGCCTGCCGGCCGAACTGGAGATCGAGTTCGAGGAATTGCGCATGGCGCTCGCCCTCGTGCAAAGCATGGACCCGCCCG
GCGTGGGTGCGCGCAGCGCGGCCGAATGCCTGGCCCTGCAGATCCGCCGCATGCCCGGCGTGCCGCTCGTCACGCGGCGC
ATGGCGCTCGTCATCGTCGAAAAATACCTGACGTGGTTCGCGCAGCGTGAATTCAACAAGCTCAAAAAGGCGCTCGATTG
CGACGACGAAGACCTGCGCGAAGCCCAGGCCGTCATCCGCCAGTGCAACCCCCATCCGGGCGCGGCGTTCGCCTCGGATG
TGTCGGACTTCGTCGTGCCCGACGTGATCGTGCGCCGCGCCAAGGACGGCTGGAGCGTGCAGCTGAATCCGGAAGTGATG
CCGCGCCTGCGCGTGAACAGCCTGTACGCCTCGCTGATGAAGCAGGGGAAATCAGAAACACAGCTGGGTGCACAGTTGCA
GGAAGCCAAGTGGCTCATCAAGAATATGCGGCAGCGCTTCGACACGATCCTGCGCGTGGCCCAGGCTATTGTGGATCGCC
AGCGCAACTTTTTCTCGCATGGCGCAGTCGCGATGCGGCCCCTTGTTTTACGTGAGATAGCTGATACACTAGGTCTACAC
GAGAGCACAATTTCTCGAGTCACAACTCAGAAATATATGCTGACCCCGCACGGCATGTTCGAGTTGAAGTATTTCTTCGG
GAGTCACGTTGCCACCGAAGCGGGAGGCGAAGCATCGTCGACGGCAATACGGGCCCTCATCGTACAACTGACAGGAGCAG
AAGACCCCAAGAATCCCTTATCCGACAGCAAGATCGCGGAGATGCTAGGGGAACAGGGAATGGTGATCGCGCGGCGCACG
GTCGCGAAGTACCGTGAGGCGCTGAAGATTCCACCCGTCAGCCTCCGCAAATCCTTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(342-497)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

58.121

100

0.595

Multiple sequence alignment