Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CS031_RS01195 Genome accession   NZ_CP024278
Coordinates   222676..223335 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain ATCC 43896     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 217676..228335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS031_RS01170 parE 217767..219659 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  CS031_RS01175 ygiN 219707..220021 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  CS031_RS01180 mdaB 220052..220633 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CS031_RS01185 ygiZ 220952..221284 (+) 333 WP_024168840.1 DUF2645 family protein -
  CS031_RS01190 qseC 221330..222679 (-) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  CS031_RS01195 ciaR 222676..223335 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  CS031_RS01200 ygiW 223487..223879 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CS031_RS01205 ygiV 223932..224414 (+) 483 WP_000183494.1 GyrI-like domain-containing protein -
  CS031_RS01215 parC 224960..227218 (+) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  CS031_RS01220 plsC 227451..228188 (+) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=212566 CS031_RS01195 WP_001221502.1 222676..223335(-) (ciaR) [Escherichia coli strain ATCC 43896]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=212566 CS031_RS01195 WP_001221502.1 222676..223335(-) (ciaR) [Escherichia coli strain ATCC 43896]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365