Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   COG40_RS17250 Genome accession   NZ_CP024275
Coordinates   3330644..3331303 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli O6:H16 strain M9682-C1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3325644..3336303
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  COG40_RS17225 ygiS 3326927..3328534 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  COG40_RS17230 mqsA 3328667..3329062 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  COG40_RS17235 mqsR 3329064..3329360 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  COG40_RS17240 ygiV 3329565..3330047 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  COG40_RS17245 ygiW 3330100..3330492 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  COG40_RS17250 ciaR 3330644..3331303 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  COG40_RS17255 qseC 3331300..3332649 (+) 1350 WP_000673409.1 quorum sensing histidine kinase QseC -
  COG40_RS17260 ygiZ 3332695..3333027 (-) 333 WP_000917686.1 DUF2645 family protein -
  COG40_RS17270 mdaB 3333346..3333927 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  COG40_RS17275 ygiN 3333958..3334272 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  COG40_RS17280 parE 3334320..3336212 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=212530 COG40_RS17250 WP_001221493.1 3330644..3331303(+) (ciaR) [Escherichia coli O6:H16 strain M9682-C1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=212530 COG40_RS17250 WP_001221493.1 3330644..3331303(+) (ciaR) [Escherichia coli O6:H16 strain M9682-C1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365