Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   A5T63_RS16785 Genome accession   NZ_CP024273
Coordinates   3263577..3264236 (+) Length   219 a.a.
NCBI ID   WP_001221488.1    Uniprot ID   -
Organism   Escherichia coli strain F9792     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3258577..3269236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A5T63_RS16760 plsC 3258723..3259460 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  A5T63_RS16765 parC 3259694..3261952 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  A5T63_RS16775 ygiV 3262498..3262980 (-) 483 WP_001718874.1 GyrI-like domain-containing protein -
  A5T63_RS16780 ygiW 3263033..3263425 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  A5T63_RS16785 ciaR 3263577..3264236 (+) 660 WP_001221488.1 quorum sensing response regulator transcription factor QseB Regulator
  A5T63_RS16790 qseC 3264233..3265582 (+) 1350 WP_000673355.1 quorum sensing histidine kinase QseC -
  A5T63_RS16795 ygiZ 3265633..3265965 (-) 333 WP_024171565.1 DUF2645 family protein -
  A5T63_RS16800 mdaB 3266323..3266904 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  A5T63_RS16805 ygiN 3266935..3267249 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  A5T63_RS16810 - 3267294..3268181 (-) 888 WP_000614954.1 MurR/RpiR family transcriptional regulator -
  A5T63_RS16815 - 3268178..3269125 (-) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24692.61 Da        Isoelectric Point: 6.3527

>NTDB_id=212446 A5T63_RS16785 WP_001221488.1 3263577..3264236(+) (ciaR) [Escherichia coli strain F9792]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQCEPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=212446 A5T63_RS16785 WP_001221488.1 3263577..3264236(+) (ciaR) [Escherichia coli strain F9792]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGTGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365