Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CCV27_RS10035 Genome accession   NZ_CP024269
Coordinates   1973381..1974040 (+) Length   219 a.a.
NCBI ID   WP_001221488.1    Uniprot ID   -
Organism   Escherichia coli strain F8111-1SC3     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1968381..1979040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCV27_RS10010 plsC 1968527..1969264 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  CCV27_RS10015 parC 1969498..1971756 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  CCV27_RS10025 ygiV 1972302..1972784 (-) 483 WP_001718874.1 GyrI-like domain-containing protein -
  CCV27_RS10030 ygiW 1972837..1973229 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CCV27_RS10035 ciaR 1973381..1974040 (+) 660 WP_001221488.1 quorum sensing response regulator transcription factor QseB Regulator
  CCV27_RS10040 qseC 1974037..1975386 (+) 1350 WP_000673355.1 quorum sensing histidine kinase QseC -
  CCV27_RS10045 ygiZ 1975437..1975769 (-) 333 WP_024171565.1 DUF2645 family protein -
  CCV27_RS10050 mdaB 1976127..1976708 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CCV27_RS10055 ygiN 1976739..1977053 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  CCV27_RS10060 - 1977098..1977985 (-) 888 WP_000614954.1 MurR/RpiR family transcriptional regulator -
  CCV27_RS10065 - 1977982..1978929 (-) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24692.61 Da        Isoelectric Point: 6.3527

>NTDB_id=212353 CCV27_RS10035 WP_001221488.1 1973381..1974040(+) (ciaR) [Escherichia coli strain F8111-1SC3]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQCEPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=212353 CCV27_RS10035 WP_001221488.1 1973381..1974040(+) (ciaR) [Escherichia coli strain F8111-1SC3]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGTGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365