Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   COG37_RS05135 Genome accession   NZ_CP024257
Coordinates   993107..993766 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli O25:H16 strain F5505-C1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 988107..998766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  COG37_RS05105 parE 988198..990090 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  COG37_RS05110 ygiN 990138..990452 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  COG37_RS05115 mdaB 990483..991064 (-) 582 WP_000072421.1 NADPH:quinone oxidoreductase MdaB -
  COG37_RS05125 ygiZ 991383..991715 (+) 333 WP_000917684.1 DUF2645 family protein -
  COG37_RS05130 qseC 991761..993110 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  COG37_RS05135 ciaR 993107..993766 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  COG37_RS05140 ygiW 993918..994310 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  COG37_RS05145 ygiV 994363..994845 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  COG37_RS05150 mqsR 995050..995178 (+) 129 Protein_943 type II toxin-antitoxin system toxin MqsR -
  COG37_RS05160 mqsR 996028..996204 (+) 177 Protein_945 type II toxin-antitoxin system toxin MqsR -
  COG37_RS05165 mqsA 996206..996601 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  COG37_RS05170 ygiS 996734..998341 (+) 1608 WP_001735743.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=212102 COG37_RS05135 WP_001221493.1 993107..993766(-) (ciaR) [Escherichia coli O25:H16 strain F5505-C1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=212102 COG37_RS05135 WP_001221493.1 993107..993766(-) (ciaR) [Escherichia coli O25:H16 strain F5505-C1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365