Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CS032_RS02190 Genome accession   NZ_CP024256
Coordinates   144779..145438 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain ATCC 43886     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 139779..150438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS032_RS02155 ygiS 140204..141811 (-) 1608 WP_001735743.1 ABC transporter substrate-binding protein -
  CS032_RS02160 mqsA 141944..142339 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  CS032_RS02165 mqsR 142341..142517 (-) 177 Protein_137 type II toxin-antitoxin system toxin MqsR -
  CS032_RS02170 - 142654..143351 (+) 698 WP_099550739.1 IS1 family transposase -
  CS032_RS02175 mqsR 143367..143495 (-) 129 Protein_139 type II toxin-antitoxin system toxin MqsR -
  CS032_RS02180 ygiV 143700..144182 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  CS032_RS02185 ygiW 144235..144627 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CS032_RS02190 ciaR 144779..145438 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  CS032_RS02195 qseC 145435..146784 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  CS032_RS02200 ygiZ 146830..147162 (-) 333 WP_000917684.1 DUF2645 family protein -
  CS032_RS02210 mdaB 147481..148062 (+) 582 WP_000072421.1 NADPH:quinone oxidoreductase MdaB -
  CS032_RS02215 ygiN 148093..148407 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  CS032_RS02220 parE 148455..150347 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=211997 CS032_RS02190 WP_001221493.1 144779..145438(+) (ciaR) [Escherichia coli strain ATCC 43886]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=211997 CS032_RS02190 WP_001221493.1 144779..145438(+) (ciaR) [Escherichia coli strain ATCC 43886]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365