Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CNZ63_RS14885 Genome accession   NZ_CP024232
Coordinates   2854280..2854939 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli O6:H16 strain 2014EL-1346-6     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2849280..2859939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNZ63_RS14855 parE 2849371..2851263 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  CNZ63_RS14860 ygiN 2851311..2851625 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  CNZ63_RS14865 mdaB 2851656..2852237 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CNZ63_RS14875 ygiZ 2852556..2852888 (+) 333 WP_000917686.1 DUF2645 family protein -
  CNZ63_RS14880 qseC 2852934..2854283 (-) 1350 WP_000673409.1 quorum sensing histidine kinase QseC -
  CNZ63_RS14885 ciaR 2854280..2854939 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  CNZ63_RS14890 ygiW 2855091..2855483 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CNZ63_RS14895 ygiV 2855536..2856018 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  CNZ63_RS14900 mqsR 2856223..2856519 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  CNZ63_RS14905 mqsA 2856521..2856916 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  CNZ63_RS14910 ygiS 2857049..2858656 (+) 1608 WP_001295629.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=211720 CNZ63_RS14885 WP_001221493.1 2854280..2854939(-) (ciaR) [Escherichia coli O6:H16 strain 2014EL-1346-6]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=211720 CNZ63_RS14885 WP_001221493.1 2854280..2854939(-) (ciaR) [Escherichia coli O6:H16 strain 2014EL-1346-6]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365