Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   A3411_RS19920 Genome accession   NZ_CP024228
Coordinates   3789247..3789906 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli O25:NM strain 2014EL-1343-2     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3784247..3794906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A3411_RS19890 parE 3784338..3786230 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  A3411_RS19895 ygiN 3786278..3786592 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  A3411_RS19900 mdaB 3786623..3787204 (-) 582 WP_000072421.1 NADPH:quinone oxidoreductase MdaB -
  A3411_RS19910 ygiZ 3787523..3787855 (+) 333 WP_000917684.1 DUF2645 family protein -
  A3411_RS19915 qseC 3787901..3789250 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  A3411_RS19920 ciaR 3789247..3789906 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  A3411_RS19925 ygiW 3790058..3790450 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  A3411_RS19930 ygiV 3790503..3790985 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  A3411_RS27810 - 3791190..3791399 (+) 210 WP_050438169.1 type II toxin-antitoxin system MqsR family toxin -
  A3411_RS19945 mqsR 3792168..3792344 (+) 177 Protein_3668 type II toxin-antitoxin system toxin MqsR -
  A3411_RS19950 mqsA 3792346..3792741 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  A3411_RS19955 ygiS 3792874..3794481 (+) 1608 WP_001735743.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=211648 A3411_RS19920 WP_001221493.1 3789247..3789906(-) (ciaR) [Escherichia coli O25:NM strain 2014EL-1343-2]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=211648 A3411_RS19920 WP_001221493.1 3789247..3789906(-) (ciaR) [Escherichia coli O25:NM strain 2014EL-1343-2]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment