Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   BUN12_RS13420 Genome accession   NZ_CP018902
Coordinates   2567262..2568647 (-) Length   461 a.a.
NCBI ID   WP_013353785.1    Uniprot ID   A0A9P1NJB2
Organism   Bacillus amyloliquefaciens strain HK1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2562262..2573647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUN12_RS13385 (BUN12_2626) flgL 2562436..2563353 (-) 918 WP_013353778.1 flagellar hook-associated protein FlgL -
  BUN12_RS13390 (BUN12_2627) flgK 2563365..2564882 (-) 1518 WP_013353779.1 flagellar hook-associated protein FlgK -
  BUN12_RS13395 (BUN12_2628) - 2564898..2565380 (-) 483 WP_013353780.1 flagellar protein FlgN -
  BUN12_RS13400 (BUN12_2629) flgM 2565395..2565661 (-) 267 WP_013353781.1 flagellar biosynthesis anti-sigma factor FlgM -
  BUN12_RS13405 (BUN12_2630) - 2565731..2566150 (-) 420 WP_013353782.1 TIGR03826 family flagellar region protein -
  BUN12_RS21725 comFC 2566224..2566913 (-) 690 WP_198315882.1 ComF family protein Machinery gene
  BUN12_RS13415 (BUN12_2632) - 2566919..2567203 (-) 285 WP_013353784.1 late competence development ComFB family protein -
  BUN12_RS13420 (BUN12_2633) comFA 2567262..2568647 (-) 1386 WP_013353785.1 DEAD/DEAH box helicase Machinery gene
  BUN12_RS13425 (BUN12_2634) - 2568754..2569602 (-) 849 WP_013353786.1 DegV family protein -
  BUN12_RS13430 (BUN12_2635) degU 2569699..2570388 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  BUN12_RS13435 (BUN12_2636) degS 2570465..2571628 (-) 1164 WP_013353787.1 two-component sensor histidine kinase DegS Regulator
  BUN12_RS13440 (BUN12_2637) - 2571851..2572498 (+) 648 WP_013353788.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52689.28 Da        Isoelectric Point: 10.2042

>NTDB_id=211549 BUN12_RS13420 WP_013353785.1 2567262..2568647(-) (comFA) [Bacillus amyloliquefaciens strain HK1]
MSGGASEFSKVRDFFYGRHLLRSEIPFSDQCIKRFIEKEYITAEPSVIRRKNRYVCRRCGQSDQARFAVFWAPSAKRHIT
YCRSCVVMGRADELTSLYSWNLAAENSWEPIKLSWEGTLTDGQKQAAEALIEAIKEKQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGSLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKRAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLEKGKLPRSVTDWV
RQKLQSRLPVFLFVPSVHILRKTTDYFQKLNIRAEGVHAEDTCRKDKVKRFREGRFDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESALVQIAGRTGRHYKHFSGDVVMFHFGMTNGMKKAKKHIEYMNKLAQKNKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=211549 BUN12_RS13420 WP_013353785.1 2567262..2568647(-) (comFA) [Bacillus amyloliquefaciens strain HK1]
ATGTCCGGAGGTGCTTCAGAATTTTCGAAGGTGCGGGATTTCTTTTACGGCAGGCATTTATTGAGATCTGAGATTCCGTT
TTCTGATCAGTGCATCAAGCGTTTTATAGAAAAAGAATATATAACGGCCGAGCCGTCAGTCATCCGCCGGAAAAACCGTT
ATGTCTGCCGCCGATGCGGCCAGTCTGACCAAGCCCGCTTTGCGGTATTTTGGGCGCCCTCTGCCAAAAGACATATTACC
TATTGCCGTTCCTGTGTCGTAATGGGAAGGGCTGATGAACTGACGTCATTATACTCTTGGAATCTAGCGGCTGAAAACAG
CTGGGAGCCTATCAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCAGGCGGCGGAAGCGCTTATAGAAGCGA
TAAAAGAGAAACAAGAGCTGCTGGTATGGGCGGTTTGCGGATCAGGGAAAACAGAGATGCTGTTTCCGGGTATAGAGTTT
GCGCTGAATCACGGCTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTTTCTGCTTTGTACGGCGGCAGTGAGGACAAAGGCAGCTTGACTCCGCTAATGATCTCAA
CCGCTCACCAGCTGATGCGCTATCGAGATATTTTTGATGTAATGATAATAGATGAAGTAGATGCTTTTCCTTTTTCAGCT
GATGAGACATTAAGATTTGCGGTTGATAAAGCCAGAAAGAAAAACAGCGCCCTAGTTTATGTAACGGCAACTCCTTCAGA
CACATTAAAAAAGAGGGCTGAGGCCGGTTTGCTGAAAAGCGTTCGCATACCGGCCAGATATCATCGGAAACCGCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTGAGAAAGGAAAACTGCCGCGTTCTGTAACGGATTGGGTC
CGGCAAAAACTTCAATCACGACTGCCGGTTTTCTTATTCGTTCCGTCTGTACATATATTAAGAAAGACAACGGATTATTT
TCAGAAATTAAACATCCGTGCAGAAGGCGTGCATGCGGAAGACACATGCAGAAAGGATAAAGTGAAACGGTTCAGAGAGG
GACGGTTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTACCTAAAGTCCAGACTTGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGCACTCGTACAGATTGCAGGCAGAACGGGCAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATGACAAACGGTATGAAGAAAGCAAAAAAACATATAGAATATATGAACAAATTGG
CACAAAAAAATAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(329-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

63.02

99.132

0.625

Multiple sequence alignment