Detailed information NTG0211535

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BUN12_RS08460
• Genome accession: NZ_CP018902
• Coordinates: 1626992..1627606 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus amyloliquefaciens strain HK1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1621992..1632606

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BUN12_RS08430 (BUN12_1667)	rpsT	1622292..1622558 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BUN12_RS08435 (BUN12_1668)	holA	1622575..1623616 (-)	1042	Protein_1667	DNA polymerase III subunit delta	-
BUN12_RS08440	-	1623656..1623810 (-)	155	Protein_1668	hypothetical protein	-
BUN12_RS08445	-	1623851..1623985 (+)	135	WP_003152870.1	YqzM family protein	-
BUN12_RS08450 (BUN12_1669)	comEC	1624004..1626355 (-)	2352	WP_041481655.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BUN12_RS08455 (BUN12_1670)	-	1626356..1626925 (-)	570	WP_003152868.1	ComE operon protein 2	-
BUN12_RS08460 (BUN12_1671)	comEA	1626992..1627606 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
BUN12_RS08465 (BUN12_1672)	comER	1627665..1628486 (+)	822	WP_013352943.1	late competence protein ComER	-
BUN12_RS08470 (BUN12_1673)	-	1628555..1629292 (-)	738	WP_013352944.1	class I SAM-dependent DNA methyltransferase	-
BUN12_RS08475 (BUN12_1674)	rsfS	1629289..1629645 (-)	357	WP_003152864.1	ribosome silencing factor	-
BUN12_RS08480 (BUN12_1675)	yqeK	1629663..1630223 (-)	561	WP_013352945.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BUN12_RS08485 (BUN12_1676)	-	1630213..1630782 (-)	570	WP_013352946.1	nicotinate-nucleotide adenylyltransferase	-
BUN12_RS08490 (BUN12_1677)	yhbY	1630793..1631083 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BUN12_RS08495 (BUN12_1678)	aroE	1631077..1631919 (-)	843	WP_013352947.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=211535 BUN12_RS08460 WP_013352942.1 1626992..1627606(-) (comEA) [Bacillus amyloliquefaciens strain HK1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=211535 BUN12_RS08460 WP_013352942.1 1626992..1627606(-) (comEA) [Bacillus amyloliquefaciens strain HK1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363