Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   CR539_RS23980 Genome accession   NZ_CP024147
Coordinates   4620386..4622467 (+) Length   693 a.a.
NCBI ID   WP_032281853.1    Uniprot ID   -
Organism   Escherichia coli strain 14EC033     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4615386..4627467
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR539_RS23960 (CR539_23865) gmk 4616604..4617227 (+) 624 WP_032281852.1 guanylate kinase -
  CR539_RS23965 (CR539_23870) rpoZ 4617282..4617557 (+) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  CR539_RS23970 (CR539_23875) spoT 4617576..4619684 (+) 2109 WP_000280473.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  CR539_RS23975 (CR539_23880) trmH 4619691..4620380 (+) 690 WP_001070174.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  CR539_RS23980 (CR539_23885) recG 4620386..4622467 (+) 2082 WP_032281853.1 ATP-dependent DNA helicase RecG Machinery gene
  CR539_RS23985 (CR539_23890) gltS 4622636..4623841 (-) 1206 WP_000468833.1 sodium/glutamate symporter -
  CR539_RS23990 (CR539_23895) xanP 4624121..4625512 (+) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  CR539_RS23995 (CR539_23900) yicH 4625633..4627342 (+) 1710 WP_032281854.1 AsmA family protein -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76588.59 Da        Isoelectric Point: 7.6532

>NTDB_id=211185 CR539_RS23980 WP_032281853.1 4620386..4622467(+) (recG) [Escherichia coli strain 14EC033]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLQTVQDLLLHLPLRYEDRTHLYPIGELLPGFYATVEGEVLNCNITFGGRRM
MTCQISDGSGILTMRFFNFNAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTNQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARVVAEIERDMALNVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRNDIIDRVRHACM
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=211185 CR539_RS23980 WP_032281853.1 4620386..4622467(+) (recG) [Escherichia coli strain 14EC033]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCGCTCAGTTCCCTAACGGGCGTTGGCGCAGCGCTTAGTAACAAGCTGGCGAA
AATCAACCTGCAGACTGTGCAGGATCTACTCTTACACCTTCCCCTGCGCTACGAAGATCGCACCCATCTCTACCCCATCG
GAGAACTACTGCCGGGCTTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCACCTTCGGCGGCCGCCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATTCTCACCATGCGCTTTTTCAACTTCAACGCGGCAATGAAAAATAGCCT
GGCGACGGGCCGCCGCGTGCTGGCCTATGGAGAAGCAAAGCGCGGTAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTGCAGGGCGATCTCAGTACGCCAGAGTTGCAGGAAACGCTCACGCCGGTTTATCCAACAACCGAAGGCGTAAAGCAG
GCCACGCTGCGTAAATTAACCAACCAGGCGCTGGATCTGCTCGACACCTGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCGCCGCCGACGCTGCAACTTAGCGATCTGG
AAACCGGGCAGCATCCGGCGCAACGTCGTCTGATTCTGGAAGAACTGCTGGCGCATAACCTCAGCATGTTAGCCTTACGC
GCCGGAGCGCAACGTTTCCATGCCCAGCCGCTGAGCGCCAATGACACGCTGAAAAATAAACTTCTTGCCGCCTTACCGTT
CAAGCCAACGGGCGCTCAGGCTCGCGTGGTGGCGGAGATCGAGCGCGATATGGCGCTGAATGTGCCGATGATGCGCCTGG
TACAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCTGCCCTCGCCGCGCTGCGTGCGATTGCCCACGGCAAACAGGTG
GCATTGATGGCACCAACCGAATTACTCGCCGAACAGCACGCCAATAACTTCCGTAACTGGTTTGCACCGCTCGGTATCGA
AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGCAAAGCGCGACTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTCGGCACCCACGCCATTTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGCTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAGGCCAGCAACAGGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCGATCCCCCGCACGCTGGCGATGACTGCGTATGCTGATCTCGATACATCAGTCATTGATGAACTGCCGCCTG
GCCGTACTCCAGTGACTACCGTTGCTATCCCGGATACACGCCGCAACGACATTATCGACCGCGTGCGCCACGCCTGCATG
ACTGAAGGGCGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCAGAGTTACTGGAAGCGCAGGCGGCGGAAGCCAC
CTGGGAAGAGTTGAAACTGGCCTTACCGGAACTGAACGTTGGTCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGG
CGGTGATGGCGTCGTTTAAACAAGGTGAGCTACACCTGCTGGTTGCCACTACCGTTATTGAAGTCGGTGTTGATGTGCCT
AACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTGCACCAGCTACGTGGGCGTGTAGGTCG
TGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTTTCTAAAACGGCGCAAATTCGCCTGCAAGTGCTGC
GCGACAGCAACGACGGTTTTGTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGTACACGTCAG
ACGGGTAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAAGTTCAGCGCTTGGCACGCCA
TATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGT
AA

Domains

Predicted by InterProScan.

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)

DEAD/DEAH box helicase (DEAD)

(273-433)

Helicase conserved C-terminal domain (Helicase_C)

(481-588)

RecG wedge domain (RecG_wedge)

(15-171)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.381

96.97

0.508
  recG/mmsA Streptococcus pneumoniae R6

38.428

99.134

0.381
  recG/mmsA Streptococcus pneumoniae R36A

38.428

99.134

0.381
  recG Bacillus subtilis subsp. subtilis str. 168

39.362

94.949

0.374

Multiple sequence alignment