Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CR539_RS20225 Genome accession   NZ_CP024147
Coordinates   3911953..3912612 (+) Length   219 a.a.
NCBI ID   WP_021574775.1    Uniprot ID   -
Organism   Escherichia coli strain 14EC033     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3906953..3917612
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CR539_RS20205 (CR539_20150) ygiS 3909158..3910765 (-) 1608 Protein_3685 ABC transporter substrate-binding protein -
  CR539_RS20210 (CR539_20155) ygiV 3910874..3911356 (-) 483 Protein_3686 GyrI-like domain-containing protein -
  CR539_RS20215 (CR539_20160) ygiW 3911409..3911801 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CR539_RS20225 (CR539_20165) ciaR 3911953..3912612 (+) 660 WP_021574775.1 quorum sensing response regulator transcription factor QseB Regulator
  CR539_RS20230 (CR539_20170) qseC 3912609..3913958 (+) 1350 WP_101969518.1 quorum sensing histidine kinase QseC -
  CR539_RS20235 (CR539_20175) mdaB 3914068..3914649 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CR539_RS20240 (CR539_20180) ygiN 3914680..3914994 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  CR539_RS20245 (CR539_20185) - 3915039..3915926 (-) 888 WP_000614954.1 MurR/RpiR family transcriptional regulator -
  CR539_RS20250 (CR539_20190) - 3915923..3916870 (-) 948 WP_101969519.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24704.67 Da        Isoelectric Point: 6.3527

>NTDB_id=211173 CR539_RS20225 WP_021574775.1 3911953..3912612(+) (ciaR) [Escherichia coli strain 14EC033]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGCQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLILKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=211173 CR539_RS20225 WP_021574775.1 3911953..3912612(+) (ciaR) [Escherichia coli strain 14EC033]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTTGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAATACTGAAACCAAAAGAATTTGCTCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365