Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BG259_RS11040 Genome accession   NZ_CP018680
Coordinates   2302050..2303066 (-) Length   338 a.a.
NCBI ID   WP_074050876.1    Uniprot ID   -
Organism   Vibrio harveyi strain QT520     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2297050..2308066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG259_RS11015 (BG259_11015) aroK 2297906..2298424 (-) 519 WP_005451507.1 shikimate kinase AroK -
  BG259_RS11020 (BG259_11020) pilQ 2298614..2300365 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  BG259_RS11025 (BG259_11025) pilP 2300400..2300915 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  BG259_RS11030 (BG259_11030) pilO 2300905..2301498 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  BG259_RS11035 (BG259_11035) pilN 2301491..2302066 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  BG259_RS11040 (BG259_11040) pilM 2302050..2303066 (-) 1017 WP_074050876.1 type IV pilus assembly protein PilM Machinery gene
  BG259_RS11045 (BG259_11045) - 2303245..2305815 (+) 2571 WP_074050877.1 penicillin-binding protein 1A -
  BG259_RS11050 (BG259_11050) oxyR 2305882..2306790 (-) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  BG259_RS11055 (BG259_11055) - 2306965..2307693 (+) 729 WP_005451494.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38065.49 Da        Isoelectric Point: 6.4206

>NTDB_id=209647 BG259_RS11040 WP_074050876.1 2302050..2303066(-) (pilM) [Vibrio harveyi strain QT520]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNIINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDSYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=209647 BG259_RS11040 WP_074050876.1 2302050..2303066(-) (pilM) [Vibrio harveyi strain QT520]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACATCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGATAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACA
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGCGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGAACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAAGTTGCTACTGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGTAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.533

100

0.905
  pilM Vibrio cholerae strain A1552

48.538

100

0.491

Multiple sequence alignment