Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   SAV_RS08295 Genome accession   NC_002758
Coordinates   1654119..1654616 (-) Length   165 a.a.
NCBI ID   WP_001788897.1    Uniprot ID   -
Organism   Staphylococcus aureus subsp. aureus Mu50     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1649119..1659616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAV_RS08270 (SAV1535) gcvPB 1649281..1650753 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  SAV_RS08275 (SAV1536) gcvPA 1650746..1652092 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  SAV_RS08280 (SAV1537) gcvT 1652112..1653203 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  SAV_RS08285 (SAV1538) - 1653362..1653886 (-) 525 WP_001015120.1 shikimate kinase -
  SAV_RS08290 - 1653876..1654022 (-) 147 WP_001792109.1 hypothetical protein -
  SAV_RS08295 (SAV1539) comGF 1654119..1654616 (-) 498 WP_001788897.1 competence type IV pilus minor pilin ComGF Machinery gene
  SAV_RS08300 (SAV1540) comGE 1654534..1654833 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  SAV_RS08305 (SAV1541) comGD 1654820..1655266 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  SAV_RS08310 (SAV1542) comGC 1655244..1655555 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  SAV_RS08315 (SAV1543) comGB 1655569..1656639 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  SAV_RS08320 (SAV1544) comGA 1656611..1657585 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  SAV_RS08325 (SAV1545) - 1657637..1658260 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  SAV_RS08330 (SAV1546) - 1658257..1658586 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  SAV_RS08335 (SAV1547) - 1658586..1659572 (-) 987 WP_000161311.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19181.99 Da        Isoelectric Point: 10.1964

>NTDB_id=20949 SAV_RS08295 WP_001788897.1 1654119..1654616(-) (comGF) [Staphylococcus aureus subsp. aureus Mu50]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=20949 SAV_RS08295 WP_001788897.1 1654119..1654616(-) (comGF) [Staphylococcus aureus subsp. aureus Mu50]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

100

100

1
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment